Anticorps Polyclonal de lapin anti-PPP2R2C

PPP2R2C Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 12747-1-AP

Synonymes

B55 GAMMA, IMYPNO, IMYPNO1, PP2A subunit B isoform B55-gamma, PP2A subunit B isoform gamma

---

Galerie de données de validation

• 

  WB analysis of HeLa using 12747-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12747-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12747-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12747-1-AP (PPP2R2C antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 12747-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12747-1-AP (PPP2R2C antibody) at dilution of 1:50 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu cérébral humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• CoIP: See 1 publications below

Informations sur le produit

12747-1-AP cible PPP2R2C dans les applications de WB, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2R2C Protéine recombinante Ag3470
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit B, gamma isoform
• Masse moléculaire calculée: 430 aa, 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC032954
• Symbole du gène: PPP2R2C
• Identification du gène (NCBI): 5522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase 2 regulatory subunit B gamma, also known as PPP2R2C or B55-gamma, encodes a gamma isoform of the subunit B55 subfamily, which is a regulatory subunit of Protein phosphatase type 2A (PP2A) (PMID: 24126060). PPP2R2C has been identified as a potential tumor suppressor in several cancers, including prostate and brain cancers (PMID: 23493267, PMID: 24126060). The decreased expression of PPP2R2C is associated with tumor progression, metastasis, and poorer patient outcomes in prostate cancer.

Publications

Species	Application	Title
mouse	WB	Protein Cell; Senktide blocks aberrant RTN3 interactome to retard memory decline and tau pathology in social isolated Alzheimer's disease mice; Authors - He-Zhou Huang
human		J Cell Sci; The role of specific PP2A complexes in the dephosphorylation of γ-H2AX.; Authors - Liping Chen
mouse	WB	J Biochem; Involvement of PP2A Methylation in the Adipogenic Differentiation of Bone Marrow Derived Mesenchymal Stem Cell.; Authors - Shunta Ikeda
mouse	WB	Biochim Biophys Acta Mol Cell Res; Hsc70 phosphorylation patterns and calmodulin regulate AP2 CCV life span for cell adhesion protein transport; Authors - G F Sengül
human	WB	Toxicol Appl Pharmacol; Chronic arsenic exposure suppresses ATM pathway activation in human keratinocytes; Authors - Alexandra N Nail
human	WB,CoIP	Cell Signal; METTL14-mediated N6-methyladenosine modification of TCP1 mRNA promotes acute myeloid leukemia progression; Authors - Mengmeng Zhang

{{ptg:RelatedPrimaryAntibodies}}