Anticorps Polyclonal de lapin anti-PPP2R3A

PPP2R3A Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 14720-1-AP

Synonymes

PPP2R3, PPP2R3A, PR130, PR72

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Galerie de données de validation

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  WB analysis of PC-3 using 14720-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 14720-1-AP (PPP2R3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 14720-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 14720-1-AP (PPP2R3A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skin using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human brain using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human brain using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lung using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human ovary using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human kidney using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human testis using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human testis using 14720-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14720-1-AP (PPP2R3A antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules PC-3, cellules MCF-7
• Résultats positifs en IHC: tissu cutané humain, tissu cérébral humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu rénal humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14720-1-AP cible PPP2R3A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2R3A Protéine recombinante Ag6450
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit B'', alpha
• Masse moléculaire calculée: 130 kDa
• Poids moléculaire observé: 130 kDa
• Numéro d’acquisition GenBank: BC065531
• Symbole du gène: PPP2R3A
• Identification du gène (NCBI): 5523
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP2R3A(Serine/threonine-protein phosphatase 2A regulatory subunit B'' subunit alpha), also named as PPP2R3, has 2 isoforms produced by alternative splicing named as PR130 and PR72. The protein contains stretches of basic residues that may be putative nuclear localization signals, and a cluster of proline residues in an N-terminal region common to both isoforms that the isoform PR130 but not PR72 is variably expressed in a number of tumor cell lines. The PR72 and PR130 cDNAs encode 72 and 130 kDa proteins when expressed in rabbit reticulocyte lysates.(PMID:8392071).

Publications

Species	Application	Title
human	WB,IF	Cell Biosci; GREM1/PPP2R3A expression in heterogeneous fibroblasts initiates pulmonary fibrosis.; Authors - Xiaoni Shi
human	WB	Cell Signal; PP2A-AMPKα-HSF1 axis regulates metal-induced HSP expression and ROS clearance.; Authors - Xiao-Nian Zhu