Anticorps Polyclonal de lapin anti-PPP2R5A

• Phare
• Validé par KD/KO

PPP2R5A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12675-2-AP

Synonymes

B56A, PP2A B subunit isoform B alpha, PPP2R5A, PR61A, PR61alpha

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Galerie de données de validation

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  WB analysis of human ileum using 12675-2-AP

  human ileum tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12675-2-AP

  WB result of PPP2R5A antibody (12675-2-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP2R5A transfected HeLa cells.

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  WB analysis of HeLa using 12675-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12675-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 12675-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12675-2-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12675-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12675-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12675-2-AP (PPP2R5A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12675-2-AP

  IP Result of anti-PPP2R5A (IP:12675-2-AP, 4ug; Detection:12675-2-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human pancreas cancer using 12675-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 12675-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 12675-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 12675-2-AP (PPP2R5A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12675-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 12675-2-AP (PPP2R5A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu d'iléon humain, cellules HeLa, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral humain, tissu pulmonaire humain, tissu rénal humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:300-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

12675-2-AP cible PPP2R5A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2R5A Protéine recombinante Ag3360
• Nom complet: protein phosphatase 2, regulatory subunit B', alpha isoform
• Masse moléculaire calculée: 486 aa, 56 kDa
• Poids moléculaire observé: 56 kDa
• Numéro d’acquisition GenBank: BC022474
• Symbole du gène: PPP2R5A
• Identification du gène (NCBI): 5525
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP2R5A(protein phosphatase 2, regulatory subunit B (B56), alpha isoform) is also named as PR61alpha and belongs to the phosphatase 2A regulatory subunit B56 family. B56 alpha subunit gene encodes a cytoplasmic phosphoprotein(PMID:7592815). The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment. This protein has 2 isoforms produced by alternative splicing with the molecular weight of 54 kDa and 50 kDa.

Publications

Species	Application	Title
human	WB,IHC	Oncol Rep; PMID: 28849187; Authors - Zehang Zhuang ; KD Validated
human	WB	Oncol Lett; PP2A promotes apoptosis and facilitates docetaxel sensitivity via the PP2A/p-eIF4B/XIAP signaling pathway in prostate cancer.; Authors - Siwen Yin
human	WB	Cell Death Dis; Direct conversion of human umbilical cord mesenchymal stem cells into retinal pigment epithelial cells for treatment of retinal degeneration; Authors - Xiaoman Zhu