Anticorps Polyclonal de lapin anti-PPP3CB-specific

• Phare
• Validé par KD/KO

PPP3CB-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 55148-1-AP

Synonymes

CALNA2, CALNB, CAM PRP catalytic subunit, CNA2, PPP3CB, PPP3CB-specific

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Galerie de données de validation

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  WB analysis using 55148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 55148-1-AP

  WB result of PPP3CB-specific antibody (55148-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPP3CB-specific transfected HEK-293 cells.

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  WB analysis using 55148-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55148-1-AP

  IP Result of anti-PPP3CB-specific (IP:55148-1-AP, 4ug; Detection:55148-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human heart using 55148-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 55148-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse kidney using 55148-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 55148-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 55148-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed A431 cells using 55148-1-AP (PPP3CB-specific antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

55148-1-AP cible PPP3CB-specific dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein phosphatase 3 (formerly 2B), catalytic subunit, beta isoform
• Masse moléculaire calculée: 59 kDa
• Poids moléculaire observé: 59 kDa
• Numéro d’acquisition GenBank: NM_021132
• Symbole du gène: PPP3CB
• Identification du gène (NCBI): 5532
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPP3CB is one of the three isoforms of the A subunit of calcineurin (CnA). Calcineurin is a calcium-dependent serine/threonine phosphatase. It consists of two subunits: subunit A (CnA) and subunit B (CnB). For CnA, three isoforms PPP3CA (CnAα), PPP3CB (CnAβ) and PPP3CC (CnAγ) have been identified. PPP3CB is a calcium-dependent, calmodulin-stimulated protein phosphatase. It may have a role in the calmodulin activation of calcineurin. This antibody is specific to PPP3CB.

Publications

Species	Application	Title
rat, mouse, human	WB,IHC,IF	J Clin Invest; MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes.; Authors - Junnan Wu
mouse	WB	Mol Psychiatry; A recurrent SHANK1 mutation implicated in autism spectrum disorder causes autistic-like core behaviors in mice via downregulation of mGluR1-IP3R1-calcium signaling.; Authors - Yue Qin
mouse	WB,IHC	J Mol Med (Berl); Angiotensin II induces calcium/calcineurin signaling and podocyte injury by downregulating microRNA-30 family members.; Authors - Yue Zhao
human	WB	Cancer Commun (Lond); HMMR alleviates endoplasmic reticulum stress by promoting autophagolysosomal activity during endoplasmic reticulum stress-driven hepatocellular carcinoma progression; Authors - Lin He