Anticorps Polyclonal de lapin anti-PPP6C

PPP6C Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15852-1-AP

Synonymes

PP6C, PPP6, PPP6C

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Galerie de données de validation

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  WB analysis using 15852-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15852-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15852-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 15852-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15852-1-AP (PPP6C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse testis using 15852-1-AP

  IP Result of anti-PPP6C (IP:15852-1-AP, 3ug; Detection:15852-1-AP 1:500) with mouse testis tissue lysate 4000ug.

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  IHC staining of mouse testis using 15852-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15852-1-AP (PPP6C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15852-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15852-1-AP (PPP6C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HEK-293, cellules NIH/3T3, tissu testiculaire de rat, tissu testiculaire humain
• Résultats positifs en IP: tissu testiculaire de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

15852-1-AP cible PPP6C dans les applications de WB, IP, IHC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP6C Protéine recombinante Ag8670
• Nom complet: protein phosphatase 6, catalytic subunit
• Masse moléculaire calculée: 305 aa, 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC006990
• Symbole du gène: PPP6C
• Identification du gène (NCBI): 5537
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,CoIP	Protein Cell; Dephosphorylation of cGAS by PPP6C impairs its substrate binding activity and innate antiviral response.; Authors - Mi Li
		J Biol Chem; Leucine Carboxyl Methyltransferase 1 (LCMT-1) Methylates Protein Phosphatase 4 (PP4) and Protein Phosphatase 6 (PP6) and Differentially Regulates the Stable Formation of Different PP4 Holoenzymes.; Authors - Juyeon Hwang
human	WB,IP	Cell Death Dis; PP6 negatively modulates LUBAC-mediated M1-ubiquitination of RIPK1 and c-FLIPL to promote TNFα-mediated cell death; Authors - Guowei Wu ; KO Validated
mouse,human	WB,IP,CoIP	Mol Cell; Energy sensor AMPK gamma regulates translation via phosphatase PPP6C independent of AMPK alpha; Authors - Qi Zhou
canine	WB	J Vet Med Sci; Protein phosphatase 6 regulates trametinib sensitivity, a mitogen-activated protein kinase kinase (MEK) inhibitor, by regulating MEK1/2-ERK1/2 signaling in canine melanoma cells; Authors - Miu Yamamoto ; KD Validated