Anticorps Monoclonal anti-PPT1

• Phare
• Validé par KD/KO

PPT1 Monoclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc

Applications

WB, IF-P, ELISA

Conjugaison

Non conjugué

CloneNo.

1A1A3

N° de cat : 67699-1-Ig

Synonymes

Palmitoyl-protein thioesterase 1, Palmitoyl-protein hydrolase 1, INCL, EC:3.1.2.22, CLN1

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Galerie de données de validation

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  WB analysis using 67699-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67699-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 67699-1-Ig

  WB result of PPT1 antibody (67699-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPT1 transfected A549 cells.

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  WB analysis of pig heart using 67699-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 67699-1-Ig (PPT1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse testis using 67699-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse testis tissue using PPT1 antibody (67699-1-Ig, Clone: 1A1A3 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, tissu cardiaque de porc, tissu cérébral de lapin, tissu cérébral de porc, tissu de cervelet de lapin, tissu de cervelet de porc
• Résultats positifs en IF-P: tissu testiculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67699-1-Ig cible PPT1 dans les applications de WB, IF-P, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc

• Réactivité: Humain, Lapin, porc
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPT1 Protéine recombinante Ag30550
• Nom complet: palmitoyl-protein thioesterase 1
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 32-34 kda
• Numéro d’acquisition GenBank: BC008426
• Symbole du gène: PPT1
• Identification du gène (NCBI): 5538
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The defective gene behind the INCL(infantile neuronal ceroid lipofuscinoses) disease, CLN1, encodes for palmitoyl protein thioesterase 1 (PPT1). It consists of 306 amino acids, including a signal sequence of 26 amino acids and three N-linked glycosylation sites. This protein exists with a molecular mass of 32 kDa, 34 kDa,36 kDa, and 38 kDa. The enzyme is transported into lysosomes of non-neuronal cells by the mannose 6-phosphate receptor (M6PR) mediated pathway and a significant amount of native PPT1 resides in a complex rather than in a monomeric form(PMID:17565660).

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