Anticorps Polyclonal de lapin anti-Progesterone Receptor

Progesterone Receptor Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (3)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(30)

N° de cat : 25871-1-AP

Synonymes

PGR, NR3C3, PR, PRGR

---

Galerie de données de validation

• 

  WB analysis of T-47D using 25871-1-AP

  T-47D cells were subjected to SDS PAGE followed by western blot with 25871-1-AP (Progesterone Receptor antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (Progesterone Receptor antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 25871-1-AP (Progesterone Receptor antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (Progesterone Receptor antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 25871-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 25871-1-AP (Progesterone Receptor antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of T-47D using 25871-1-AP

  Immunofluorescent analysis of (4% PFA) fixed T-47D cells using Progesterone Receptor antibody (25871-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules T-47D,
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules T-47D,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 9 publications below
• IHC: See 20 publications below
• IF: See 3 publications below

Informations sur le produit

25871-1-AP cible Progesterone Receptor dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Chèvre, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Progesterone Receptor Protéine recombinante Ag22986
• Nom complet: progesterone receptor
• Masse moléculaire calculée: 933 aa, 99 kDa
• Poids moléculaire observé: 80 kDa, 110-115 kDa
• Numéro d’acquisition GenBank: NM_000926
• Symbole du gène: Progesterone Receptor
• Identification du gène (NCBI): 5241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGR (Progesterone Receptor), also named as NR3C3 and PR, belongs to the nuclear hormone receptor family and NR3 subfamily. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues. Progesterone receptor isoform B (PRB) is involved activation of c-SRC/MAPK signaling on hormone stimulation. The ER and PR status has been used as a predictor of breast carcinoma responsiveness to endocrine therapy and as a prognostic indicator for early recurrence. This antibody recognizes both PR-A and PR-B.

Publications

Species	Application	Title
human	WB	Nat Commun; Glucocorticoid receptor regulates PD-L1 and MHC-I in pancreatic cancer cells to promote immune evasion and immunotherapy resistance.; Authors - Yalan Deng
human	IF,WB	Nucleic Acids Res; ZNF598 co-translationally titrates poly(GR) protein implicated in the pathogenesis of C9ORF72-associated ALS/FTD; Authors - Jumin Park
human	IHC	Acta Neuropathol; Increased prevalence of granulovacuolar degeneration in C9orf72 mutation.; Authors - Yuichi Riku
pig	IHC	J Anim Sci Biotechnol; Dietary supplementation with N-acetyl-L-cysteine ameliorates hyperactivated ERK signaling in the endometrium that is linked to poor pregnancy outcomes following ovarian stimulation in pigs; Authors - Linghua Cheng
goat	WB,IHC	Int J Mol Sci; The High Level of RANKL Improves IκB/p65/Cyclin D1 Expression and Decreases p-Stat5 Expression in Firm Udder of Dairy Goats; Authors - Zhen Gao
rat	WB,IHC	Front Endocrinol (Lausanne); Effect of acupuncture on the opening time of implantation window and endometrial receptivity in controlled ovarian hyperstimulation rats during peri-implantation period; Authors - Runan Hu

{{ptg:RelatedPrimaryAntibodies}}