Anticorps Polyclonal de lapin anti-PRAP1

• Phare
• Validé par KD/KO

PRAP1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 11932-1-AP

Synonymes

PRAP1, PRO1195, proline rich acidic protein 1, RP11 122K13.6, UPA

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Galerie de données de validation

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  WB analysis of L02 using 11932-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 11932-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 11932-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 11932-1-AP (PRAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 11932-1-AP (PRAP1 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse small intestine using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse small intestine using 11932-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 11932-1-AP (PRAP1 Antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules L02, tissu pancréatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu d'intestin grêle de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

11932-1-AP cible PRAP1 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRAP1 Protéine recombinante Ag2526
• Nom complet: proline-rich acidic protein 1
• Masse moléculaire calculée: 151 aa, 17 kDa
• Poids moléculaire observé: 16-20 kDa
• Numéro d’acquisition GenBank: BC029447
• Symbole du gène: PRAP1
• Identification du gène (NCBI): 118471
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proline rich acidic protein 1 (PRAP1) is also named as PRO1195 or UPA. It may play an important role in maintaining normal growth homeostasis in epithelial cells. Mitotic arrest deficient 1 (MAD1) is a key component in mitotic checkpoint signalling. Recent study identified PRAP1 as a novel MAD1 binding partner using yeast-two hybrid screening (PMID: 24374861). PRAP1 has four isoforms with MW 16-20 kDa and 10 kDa.

Publications

Species	Application	Title
human	IHC,WB	Adv Sci (Weinh); Adipocyte-Derived Exosomal MTTP Suppresses Ferroptosis and Promotes Chemoresistance in Colorectal Cancer; Authors - Qiumo Zhang ; KD Validated
human,mouse	WB,IF	Nat Commun; Single-cell RNA sequencing reveals placental response under environmental stress; Authors - Eric Van Buren
human	WB	Research (Wash D C); Mesenchymal Stem Cells Carrying Viral Fusogenic Protein p14 to Treat Solid Tumors by Inducing Cell-Cell Fusion and Immune Activation; Authors - Yao Wang
human	IF	Elife; scRNA-seq and scATAC-seq reveal that Sertoli cell mediates spermatogenesis disorders through stage-specific communications in non-obstructive azoospermia; Authors - Shimin Wang

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