Anticorps Polyclonal de lapin anti-PRC1

• Phare
• Validé par KD/KO

PRC1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 15617-1-AP

Synonymes

ASE1, PRC1

---

Galerie de données de validation

• 

  WB analysis of MCF-7 using 15617-1-AP

  WB result of PRC1 antibody (15617-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRC1 transfected MCF-7 cells.

• 

  WB analysis using 15617-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15617-1-AP (PRC1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 15617-1-AP

  IP Result of anti-PRC1 (IP:15617-1-AP, 4ug; Detection:15617-1-AP 1:300) with HEK-293 cells lysate 1480ug.

• 

  IHC staining of human breast cancer using 15617-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15617-1-AP (PRC1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15617-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15617-1-AP (PRC1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 15617-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRC1 antibody (15617-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules C6, cellules HEK-293, cellules HeLa, cellules K-562, cellules Raji, cellules SH-SY5Y
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 10 publications below
• IHC: See 4 publications below
• IF: See 9 publications below

Informations sur le produit

15617-1-AP cible PRC1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRC1 Protéine recombinante Ag8028
• Nom complet: protein regulator of cytokinesis 1
• Masse moléculaire calculée: 72 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC003138
• Symbole du gène: PRC1
• Identification du gène (NCBI): 9055
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRC1 (protein regulator of cytokinesis 1) is a microtubule-bundling protein that is involved in cytokinesis. Depletion of PRC1 leads to failed cytokinesis. PRC1 is located in the nucleus during interphase, and becomes associated with mitotic spindles in a highly dynamic manner during mitosis, and localizes to the cell mid-body during cytokinesis. PRC1 is a substrate of CDK1 and it was assumed that CDK1 phosphorylation blocks its activity during metaphase. Three isoforms of PRC1 exist due to the alternative splicing, with predictive molecular weight of 71.6 kDa, 70.2 kDa and 66.2 kDa, respectively. The dual bands between 66-72 kDa detected by this antibody may represent different forms of PRC1.

Publications

Species	Application	Title
human	WB,IHC	Nat Commun; Therapeutic targeting of the PLK1-PRC1-axis triggers cell death in genomically silent childhood cancer.; Authors - Jing Li
mouse	IF	Dev Cell; Distinct classes of lagging chromosome underpin age-related oocyte aneuploidy in mouse.; Authors - Aleksandar I Mihajlović
human	WB	Biomaterials; Biocompatible PEGylated Gold nanorods function As cytokinesis inhibitors to suppress angiogenesis.; Authors - Hongyuan Song
human	IF	PLoS Biol; The ciliopathy protein CCDC66 controls mitotic progression and cytokinesis by promoting microtubule nucleation and organization.; Authors - Umut Batman
mouse	WB	Development; Aurora kinase B inhibits aurora kinase A to control maternal mRNA translation in mouse oocytes.; Authors - Mansour Aboelenain
human	WB,IHC	Front Cell Dev Biol; PRC1 and RACGAP1 are Diagnostic Biomarkers of Early HCC and PRC1 Drives Self-Renewal of Liver Cancer Stem Cells.; Authors - Shixin Liao