Anticorps Polyclonal de lapin anti-PREB

• Phare
• Validé par KD/KO

PREB Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 10146-2-AP

Synonymes

SEC12, Prolactin regulatory element-binding protein, Guanine nucleotide-exchange factor SEC12

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Galerie de données de validation

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  WB analysis of SKOV-3 using 10146-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 cells using 10146-2-AP

  WB result of anti-PREB (10146-2-AP, 1:250) with HepG2 and HT1080 by Dr. Dzmitry Batrakou.

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  WB analysis of HepG2 using 10146-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HT-1080 using 10146-2-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 10146-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SKOV-3 using 10146-2-AP

  SKOV-3 cells were subjected to SDS PAGE followed by western blot with 10146-2-AP (PREB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of SKOV-3 using 10146-2-AP

  IP result of anti-PREB (IP:10146-2-AP, 4ug; Detection:10146-2-AP 1:500) with SKOV-3 cells lysate 3200ug.

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  IHC staining of human prostate cancer using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse intestine using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse intestine tissue slide using 10146-2-AP (PREB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 10146-2-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 10146-2-AP (PREB Antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of HepG2 using 10146-2-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PREB antibody (10146-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SKOV-3, cellules 3T3-L1, cellules HepG2, cellules HT-1080
• Résultats positifs en IP: cellules SKOV-3
• Résultats positifs en IHC: mouse intestine, tissu de cancer de la prostate humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

10146-2-AP cible PREB dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PREB Protéine recombinante Ag0202
• Nom complet: prolactin regulatory element binding
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 45 kDa
• Numéro d’acquisition GenBank: BC002765
• Symbole du gène: PREB
• Identification du gène (NCBI): 10113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PREB contains two PQ-rich potential transactivation domains, but no apparent DNA-binding motif, and exhibits sequence-specific binding to Pit1-binding element of the prolactin (PRL) promoter. It may act as a transcriptional regulator and is thought to be involved in some of the developmental abnormalities observed in patients with partial trisomy 2p.

Publications

Species	Application	Title
human	WB	EMBO J; The unfolded protein response regulates ER exit sites via SNRPB-dependent RNA splicing and contributes to bone development; Authors - Muhammad Zahoor
mouse,human	WB	Redox Biol; Selenoprotein K contributes to CD36 subcellular trafficking in hepatocytes by accelerating nascent COPII vesicle formation and aggravates hepatic steatosis; Authors - Mengyue You
	IP,WB,IF	Int J Biol Macromol; Cellular OCIAD2 protein is a proviral factor for hepatitis C virus replication.; Authors - Zibing Yang
human	WB,CoIP	J Cell Sci; A Wntless-SEC12 complex on the ER membrane regulates early Wnt secretory vesicle assembly and mature ligand export.; Authors - Jiaxin Sun ; KD Validated
human	WB	Biochem Biophys Res Commun; Pull down assay for GTP-bound form of Sar1a reveals its activation during morphological differentiation.; Authors - Yuri Urai
mouse	WB	Biochim Biophys Acta; Prolactin regulatory element-binding (PREB) protein regulates hepatic glucose homeostasis.; Authors - Joo-Man Park

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