Anticorps Polyclonal de lapin anti-PRICKLE1

• Phare
• Validé par KD/KO

PRICKLE1 Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (2)

Applications

WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 22589-1-AP

Synonymes

EPM1B, Prickle-like protein 1, REST/NRSF-interacting LIM domain protein 1, RILP

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Galerie de données de validation

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  WB analysis of mouse brain using 22589-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 22589-1-AP (PRICKLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Neuro-2a using 22589-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 22589-1-AP (PRICKLE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 22589-1-AP

  IP result of anti-PRICKLE1 (IP:22589-1-AP, 3ug; Detection:22589-1-AP 1:1000) with mouse brain tissue lysate 7000ug.

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  IHC staining of mouse cerebellum using 22589-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22589-1-AP (PRICKLE1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 22589-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 22589-1-AP (PRICKLE1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 22589-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PRICKLE1 antibody (22589-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse cerebellum using 22589-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using PRICKLE1 antibody (22589-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HeLa using 22589-1-AP

  1x10^6 HeLa cells were intracellularly stained with 0.8 ug Anti-Human PRICKLE1 (22589-1-AP)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules Neuro-2a
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cervelet de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cervelet de souris,
• Résultats positifs en FC (Intra): cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IF: See 6 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

22589-1-AP cible PRICKLE1 dans les applications de WB, IHC, IF-P, FC (Intra), IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRICKLE1 Protéine recombinante Ag18407
• Nom complet: prickle homolog 1 (Drosophila)
• Masse moléculaire calculée: 831 aa, 94 kDa
• Poids moléculaire observé: 94 kDa, 55-60 kDa
• Numéro d’acquisition GenBank: BC114939
• Symbole du gène: PRICKLE1
• Identification du gène (NCBI): 144165
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRICKLE1, also known as RILP, EPM1B, encodes a cytoplasmic protein with an N-terminal PET (Pk, Espinas, Testin) domain, three LIM domains and a C-terminal PKH (prickle homologous) domain. PRICKLE1 is expressed at the highest levels in placenta and lower levels in lung, liver, kidney and pancreas(PMID: 12525887). PRICKLE1 localizes to the nuclear membrane and has been implicated in the nuclear trafficking of the transcription repressors REST/NRSF and REST4. Mutations in PRICKLE1 have been linked to progressive myoclonus epilepsy(PMID: 18976727).

Publications

Species	Application	Title
mouse	WB	Sci Adv; Prickle promotes the formation and maintenance of glutamatergic synapses by stabilizing the intercellular planar cell polarity complex.; Authors - Yue Ban ; KO Validated
chicken	WB	Development; Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.; Authors - Jianqiong Lin
		Glia; The formation of paranodal spirals at the ends of CNS myelin sheaths requires the planar polarity protein Vangl2.; Authors - Andrew A Jarjour
mouse	WB	Exp Neurol; Mutation of the murine Prickle1 (R104Q) causes phenotypes analogous to human symptoms of epilepsy and autism.; Authors - Yue Ban
human	WB	J Transl Med; PRICKLE1, a Wnt/PCP signaling component, is overexpressed and associated with inferior prognosis in acute myeloid leukemia.; Authors - Duanfeng Jiang
	IF	bioRxiv; Differential Sensitivity of Midline Patterning to Mitosis during and after Primitive Streak Extension; Authors - Zhiling Zhao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Murali (Verified Customer) (10-31-2022)

I will recommend this antibody for IF.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1 to 200

PRICKLE1 Antibody Immunofluorescence validation (1 to 200 dilution) in (Cat no:22589-1-AP)

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