Anticorps Polyclonal de lapin anti-PRKACB

PRKACB Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 12232-1-AP

Synonymes

PKA C beta, PKACB, PRKACB

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Galerie de données de validation

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  WB analysis of MCF-7 using 12232-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 12232-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12232-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 12232-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 12232-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12232-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 12232-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12232-1-AP (PRKACB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 12232-1-AP

  IP Result of anti-PRKACB (IP:12232-1-AP, 4ug; Detection:12232-1-AP 1:500) with mouse brain tissue lysate 2640ug.

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  IHC staining of mouse brain using 12232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12232-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12232-1-AP (PRKACB antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human medulloblastoma using 12232-1-AP

  Immunohistochemical analysis of paraffin-embedded human medulloblastoma using 12232-1-AP (PRKACB antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HeLa using 12232-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRKACB antibody (12232-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules A549, cellules HT-1080, cellules PC-3, tissu cardiaque de souris, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de médulloblastome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

12232-1-AP cible PRKACB dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKACB Protéine recombinante Ag2870
• Nom complet: protein kinase, cAMP-dependent, catalytic, beta
• Masse moléculaire calculée: 41 kDa, 46 kDa
• Poids moléculaire observé: 36-55 kDa
• Numéro d’acquisition GenBank: BC016285
• Symbole du gène: PRKACB
• Identification du gène (NCBI): 5567
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRKACB, also named as PKA C-beta, belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family and cAMP subfamily. It mediates cAMP-dependent signaling triggered by receptor binding to GPCRs. PKA activation regulates diverse cellular processes such as cell proliferation, the cell cycle, differentiation and regulation of microtubule dynamics, chromatin condensation and decondensation, nuclear envelope disassembly and reassembly, as well as regulation of intracellular transport mechanisms and ion flux.

Publications

Species	Application	Title
mouse	WB	Int J Nanomedicine; Pseudoephedrine Nanoparticles Alleviate Adriamycin-Induced Reproductive Toxicity Through the GnRhR Signaling Pathway.; Authors - Yang Fu
mouse,rat	WB	Biomed Pharmacother; Ephedra herb reduces adriamycin-induced testicular toxicity by upregulating the gonadotropin-releasing hormone signalling pathway.; Authors - Yang Fu
human	WB	Biomed Res Int; MicroRNA-384 Inhibits the Progression of Papillary Thyroid Cancer by Targeting PRKACB.; Authors - Yongxia Wang
human	WB	Int J Mol Sci; Dynamic Alteration Profile and New Role of RNA m6A Methylation in Replicative and H2O2-Induced Premature Senescence of Human Embryonic Lung Fibroblasts; Authors - Fan Wu
human	IHC	Front Med (Lausanne); A glycometabolic gene signature associating with immune infiltration and chemosensitivity and predicting the prognosis of patients with osteosarcoma; Authors - Fengyan Wang
mouse	WB,IF,IP	CNS Neurosci Ther; Inhibition of ANXA2 activity attenuates epileptic susceptibility and GluA1 phosphorylation; Authors - Limin Ma