Anticorps Polyclonal de lapin anti-AMPK Gamma 2

AMPK Gamma 2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(2)

N° de cat : 12568-1-AP

Synonymes

AMPK gamma2, PRKAG2, 5'-AMP-activated protein kinase subunit gamma-2, AAKG, AAKG2

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Galerie de données de validation

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  WB analysis using 12568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis using 12568-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12568-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse eye using 12568-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 12568-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 12568-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 12568-1-AP (AMPK gamma2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary tumor using 12568-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 12568-1-AP (AMPK Gamma 2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Neuro-2a, cellules PC-3, tissu oculaire de souris, tissus cardiaques de rat, tissus cardiaques de souris
• Résultats positifs en IHC: tissu d'estomac humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

12568-1-AP cible AMPK Gamma 2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: AMPK Gamma 2 Protéine recombinante Ag3214
• Nom complet: protein kinase, AMP-activated, gamma 2 non-catalytic subunit
• Masse moléculaire calculée: 569 aa, 63 kDa
• Poids moléculaire observé: 63-70 kDa, 38 kDa
• Numéro d’acquisition GenBank: BC020540
• Symbole du gène: PRKAG2
• Identification du gène (NCBI): 51422
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

AMP-activated protein kinase (AMPK) is a heterotrimeric protein composed of a catalytic alpha subunit, a noncatalytic beta subunit, and a noncatalytic regulatory gamma subunit. Various forms of each of these subunits exist, encoded by different genes. AMPK is an important energy-sensing enzyme that monitors cellular energy status and functions by inactivating key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. AMPK gamma2 is a member of the AMPK gamma subunit family. Mutations in this gene have been associated with Wolff-Parkinson-White syndrome, familial hypertrophic cardiomyopathy, and glycogen storage disease of the heart.

Publications

Species	Application	Title
human	WB	J Med Chem; Identification of Pyruvate Carboxylase as the Cellular Target of Natural Bibenzyls with Potent Anticancer Activity against Hepatocellular Carcinoma via Metabolic Reprogramming.; Authors - Yuwen Sheng
human	WB,IF	J Cardiol; Identification and functional analysis of a novel PRKAG2 mutation responsible for Chinese PRKAG2 cardiac syndrome reveal an important role of non-CBS domains in regulating the AMPK pathway.; Authors - Zhang Bi-Li BL

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