Anticorps Polyclonal de lapin anti-PKC Delta

• Phare
• Validé par KD/KO

PKC Delta Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (2)

N° de cat : 14188-1-AP

Synonymes

PRKCD, EC:2.7.10.2, EC:2.7.11.13, MAY1, nPKC-delta

---

Galerie de données de validation

• 

  WB analysis using 14188-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14188-1-AP (PKC Delta antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 14188-1-AP

  IP result of anti-PKC Delta (IP:14188-1-AP, 4ug; Detection:14188-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.

• 

  IHC staining of rat stomach using 14188-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 14188-1-AP (PKC Delta antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 14188-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 14188-1-AP (PKC Delta antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of Saos-2 using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Saos-2 cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

• 

  IF Staining of A431 using 14188-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PKC Delta antibody (14188-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules C6, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: human ovary cancer tissue, tissu d'estomac de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: Saos-2 cells, cellules A431, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14188-1-AP cible PKC Delta dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PKC Delta Protéine recombinante Ag5473
• Nom complet: protein kinase C, delta
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 70-80 kDa
• Numéro d’acquisition GenBank: BC043350
• Symbole du gène: PKC Delta
• Identification du gène (NCBI): 5580
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PKC Delta is a member of the protein kinase C family of serine- and threonine-specific protein kinases. The encoded protein is activated by diacylglycerol and is both a tumor suppressor and a positive regulator of cell cycle progression. Also, this protein can positively or negatively regulate apoptosis. Defects in this gene are a cause of autoimmune lymphoproliferative syndrom.

Publications

Species	Application	Title
human	WB	Nat Commun; Discovery of a signaling feedback circuit that defines interferon responses in myeloproliferative neoplasms.; Authors - Diana Saleiro ; KD Validated
mouse	WB	JCI Insight; Targeting ATGL to rescue BSCL2 lipodystrophy and its associated cardiomyopathy.; Authors - Hongyi Zhou
rat	WB	Mol Cancer Res; PKCð regulates chromatin remodeling and DNA repair through SIRT6; Authors - Trisiani Affandi
human	WB	J Biol Chem; Tyrosine kinase inhibitors protect the salivary gland from radiation damage by increasing DNA double strand break repair.; Authors - Trisiani Affandi
mouse,human	WB,IHC	Food Funct; Theaflavin-3,3'-di-gallate represses prostate cancer by activating the PKCδ/aSMase signaling pathway through a 67 kDa laminin receptor.; Authors - Lingli Sun
human	WB	Chem Biol Interact; ZCL-082, a boron-containing compound, induces apoptosis of non-Hodgkin's lymphoma via targeting p90 ribosomal S6 kinase 1/NF-κB signaling pathway.; Authors - Chunmin Ma

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Wassim (Verified Customer) (04-23-2019)

This antibody picks a band ~76 KDa in mouse kidney tubular cells lysed in RIPA buffer.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Kidney Epithelial Cells

PKC Delta Antibody Western Blot, validation (1:3000 dilution) in Mouse Kidney Epithelial Cells (Cat no:14188-1-AP)

FH

Angela (Verified Customer) (12-17-2018)

Lanes are as follows: 1 SCR control2 pkcd shrna13 pkcd shrna24 pkcd shrna35 50uM etoposide 18hr6 untreated control

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Salivary cell line

PKC Delta Antibody Western Blot, validation (1:1000 dilution) in Salivary cell line (Cat no:14188-1-AP)

{{ptg:RelatedPrimaryAntibodies}}