Anticorps Polyclonal de lapin anti-PRKCDBP

• Phare
• Validé par KD/KO

PRKCDBP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(26)

N° de cat : 16250-1-AP

Synonymes

Caveolae-associated protein 3, Cavin 3, Cavin-3, hSRBC, Protein kinase C delta-binding protein

---

Galerie de données de validation

• 

  WB analysis of HeLa using 16250-1-AP

  WB result of PRKCDBP antibody (16250-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRKCDBP transfected HeLa cells.

• 

  WB analysis using 16250-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16250-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 16250-1-AP (PRKCDBP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 16250-1-AP

  IP result of anti-PRKCDBP (IP:16250-1-AP, 4ug; Detection:16250-1-AP 1:1000) with A549 cells lysate 1120 ug.

• 

  IHC staining of human colon using 16250-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal colon slide using 16250-1-AP (PRKCDBP antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of MCF-7 using 16250-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PRKCDBP antibody 16250-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules L02, cellules HeLa, cellules HSC-T6, cellules MCF-7, tissu pulmonaire de rat, tissu pulmonaire de souris
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: human normal colon, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 21 publications below
• IHC: See 4 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

16250-1-AP cible PRKCDBP dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRKCDBP Protéine recombinante Ag9209
• Nom complet: protein kinase C, delta binding protein
• Masse moléculaire calculée: 261 aa, 28 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC011585
• Symbole du gène: PRKCDBP
• Identification du gène (NCBI): 112464
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein kinase C delta-binding protein (PRKCDBP) is a putative tumor suppressor in which alteration has been observed in several human cancers. It is a BRCA1-interacting protein, and may also be involved in DNA damage response and participate in BRCA1-mediated tumor suppression pathways. PKCdelta binds to the C-terminal half of PRKCDBP through the regulatory domain and phosphorylates it in vitro. In COS1 cells, the phosphorylation of over-expressed PRKCDBP is stimulated by 12-O-tetradecanoylphorbol-13-acetate and further enhanced by the over-expression of PKCdelta

Publications

Species	Application	Title
human	WB	ACS Nano; Engineering Extracellular Vesicles Restore the Impaired Cellular Uptake and Attenuate Intervertebral Disc Degeneration.; Authors - Zhiwei Liao
mouse	WB	PLoS Biol; Endocytic crosstalk: cavins, caveolins, and caveolae regulate clathrin-independent endocytosis.; Authors - Natasha Chaudhary
human	WB,IF	Nat Commun; Identification of intracellular cavin target proteins reveals cavin-PP1alpha interactions regulate apoptosis.; Authors - Kerrie-Ann McMahon ; KD Validated
human	WB,IP,IF	Dev Cell; Caveolae sense oxidative stress through membrane lipid peroxidation and cytosolic release of CAVIN1 to regulate NRF2; Authors - Yeping Wu
mouse	WB,IF	Elife; Cavin-3 dictates the balance between ERK and Akt signaling.; Authors - Victor J Hernandez
mouse	WB	Elife; Deciphering caveolar functions by syndapin III KO-mediated impairment of caveolar invagination.; Authors - Eric Seemann ; KO Validated

{{ptg:RelatedPrimaryAntibodies}}