Anticorps Polyclonal de lapin anti-PRKG2

• Phare
• Validé par KD/KO

PRKG2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 55138-1-AP

Synonymes

cGK2, cGKII, cGMP dependent protein kinase 2, cGMP-dependent protein kinase 2, cGMP-dependent protein kinase II

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Galerie de données de validation

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  WB analysis using 55138-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 55138-1-AP (PRKG2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 55138-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55138-1-AP (PRKG2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 55138-1-AP

  IP result of anti-PRKG2 (IP:55138-1-AP, 4ug; Detection:55138-1-AP 1:500) with mouse brain tissue lysate 4000ug.

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  IHC staining of human colon using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human colon using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lung using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung using 55138-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 55138-1-AP (PRKG2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of C6 using 55138-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed C6 cells using PRKG2 antibody (55138-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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  IF Staining of C6 using 55138-1-AP

  Immunofluorescent analysis of C6 cells using 55138-1-AP (PRKG2 antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de côlon humain, tissu pulmonaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C6,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

55138-1-AP cible PRKG2 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein kinase, cGMP-dependent, type II
• Masse moléculaire calculée: 87 kDa
• Poids moléculaire observé: 85-90 kDa
• Numéro d’acquisition GenBank: NM_006259
• Symbole du gène: PRKG2
• Identification du gène (NCBI): 5593
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRKG2, also named as PRKGR2 and cGK2, belongs to the protein kinase superfamily, AGC Ser/Thr protein kinase family and cGMP subfamily. It catalyses the reaction: ATP + a protein = ADP + a phosphoprotein. The antibody is specific to PRKG2.

Publications

Species	Application	Title
human	WB,IHC	Oncogene; Transcriptional reprogramming triggered by neonatal UV radiation or Lkb1 loss prevents BRAFV600E-induced growth arrest in melanocytes; Authors - Kimberley McGrail
mouse	WB	EMBO Rep; Phosphorylation of myelin regulatory factor by PRKG2 mediates demyelination in Huntington's disease.; Authors - Peng Yin ; KO Validated
human	WB	Front Oncol; High-Risk HPV16 E6 Activates the cGMP/PKG Pathway Through Glycosyltransferase ST6GAL1 in Cervical Cancer Cells.; Authors - Jun Wang
human	WB	Mol Cell Endocrinol; EDNRB negatively regulates glycolysis to exhibit anti-tumor functions in prostate cancer by cGMP/PKG pathway; Authors - Xun Li
human	WB	Nutr Cancer; Erianin Exerts Antineoplastic Effects on Esophageal Squamous Cell Carcinoma Cells by Activating the cGMP-PKG Signaling Pathway; Authors - Xin Deng
human	WB	Cell Death Dis; The novel ZEB1-upregulated protein PRTG induced by Helicobacter pylori infection promotes gastric carcinogenesis through the cGMP/PKG signaling pathway.; Authors - Tian Xiang

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