Anticorps Polyclonal de lapin anti-PRM1

PRM1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 15697-1-AP

Synonymes

P1, Cysteine-rich protamine, CT94.1

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Galerie de données de validation

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  WB analysis using 15697-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 15697-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 15697-1-AP

  rat testis tissue were subjected to SDS PAGE followed by western blot with 15697-1-AP (PRM1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain, tissu testiculaire de rat, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

15697-1-AP cible PRM1 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRM1 Protéine recombinante Ag8323
• Nom complet: PRM1
• Masse moléculaire calculée: 51 aa, 6 kDa
• Poids moléculaire observé: 13-20 kDa
• Numéro d’acquisition GenBank: BC003673
• Symbole du gène: PRM1
• Identification du gène (NCBI): 5619
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRM1 is a 51 amino acid protein. PRM1 substitutes for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823)

Publications

Species	Application	Title
mouse	WB	Environ Pollut; Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice.; Authors - Jianhui Liu
mouse	WB	Int J Mol Sci; BAF-L Modulates Histone-to-Protamine Transition during Spermiogenesis.; Authors - Chao Huang
mouse	WB	Theriogenology; Eif2s3y alleviated LPS-induced damage to mouse testis and maintained spermatogenesis by negatively regulating Adamts5; Authors - Yunxiang Li
mouse	WB	Reprod Sci; Sertoli Cell-Conditioned Medium Induces Differentiation of Bone Marrow-Derived Mesenchymal Stem Cells to Male Germ-Like Cells in Busulfan-Induced Azoospermic Mouse Model; Authors - Nasrin Khanmohammadi
mouse	WB	Eur J Pharmacol; 5- methylcytidine effectively improves spermatogenesis recovery in busulfan-induced oligoasthenospermia mice; Authors - Chengniu Wang
human	IF	Cell Rep Med; Decoding the pathogenesis of spermatogenic failure in cryptorchidism through single-cell transcriptomic profiling; Authors - Xiaoyan Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Chongil (Verified Customer) (08-19-2021)

Prm1 antibody may detect a wrong protein. Prm1 is detected in elongatid spermatids and testicular sperm heads. However, this antibody detected in spermatocytes, round spermatids, and sperm tails but not in sperm heads.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Mouse testis section

PRM1 Antibody Immunofluorescence validation (1:300 dilution) in Mouse testis section (Cat no:15697-1-AP)

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