Anticorps Polyclonal de lapin anti-PRMT1

• Phare
• Validé par KD/KO

PRMT1 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 11279-1-AP

Synonymes

ANM1, HCP1, HMT2, HRMT1L2, IR1B4, PRMT1

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Galerie de données de validation

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  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 11279-1-AP

  WB result of PRMT1 antibody (11279-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT1 transfected A549 cells.

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  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11279-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 11279-1-AP

  IP result of anti-PRMT1(IP:11279-1-AP, 4ug; Detection:11279-1-AP 1:2000) with A549 cells lysate 920 ug.

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  IHC staining of human colon using 11279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 11279-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 11279-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (11279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of Raji using 11279-1-AP

  1X10^6 Raji cells were stained with 0.2ug PRMT1 antibody (11279-1-AP, red) and control antibody (blue). Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100. Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: cellules A549,
• Résultats positifs en IHC: tissu de côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,
• Résultats positifs en cytométrie: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

11279-1-AP cible PRMT1 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRMT1 Protéine recombinante Ag1790
• Nom complet: protein arginine methyltransferase 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 40-42 kDa
• Numéro d’acquisition GenBank: BC019268
• Symbole du gène: PRMT1
• Identification du gène (NCBI): 3276
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Cell Metab; Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners; Authors - Qing Li
human	IHC	Oncogene; PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.; Authors - Xin-Ke Yin
human,mouse	WB,IHC	NPJ Precis Oncol; The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer; Authors - Xiangwei Zeng
mouse	WB	Diabetes; PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ; Authors - Yi Zhong
mouse	WB,IF	Front Mol Neurosci; Cingulate protein arginine methyltransferases 1 regulates peripheral hypersensitivity via fragile X messenger ribonucleoprotein; Authors - Cheng Wu ; KD Validated
human	WB	Toxicol Appl Pharmacol; Ribavirin inhibits the growth and ascites formation of hepatocellular carcinoma through downregulation of type I CARM1 and type II PRMT5; Authors - Yizhen Tian

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HUVECs