Anticorps Polyclonal de lapin anti-PROX1

• Phare
• Validé par KD/KO

PROX1 Polyclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IF, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

42

Avis (1)

N° de cat : 11067-2-AP

Synonymes

prospero homeobox 1, Prospero homeobox protein 1, PROX 1, PROX1

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Galerie de données de validation

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  WB analysis using 11067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11067-2-AP (PROX1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11067-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11067-2-AP (PROX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 11067-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11067-2-AP (PROX1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11067-2-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11067-2-AP (PROX1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 11067-2-AP

  Immunofluorescent analysis of HepG2 cells, using PROX1 antibody 11067-2-AP at 1:100 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 11067-2-AP

  1X10^6 HepG2 cells were stained with 0.2ug PROX1 antibody (11067-2-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules HuH-7, cellules MCF-7, tissu hépatique de rat, tissu hépatique de souris, tissu hépatique humain
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 6 publications below
• WB: See 21 publications below
• IF: See 16 publications below
• IP: See 1 publications below
• FC: See 1 publications below
• ChIP: See 6 publications below

Informations sur le produit

11067-2-AP cible PROX1 dans les applications de WB, IP, IF, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PROX1 Protéine recombinante Ag1543
• Nom complet: prospero homeobox 1
• Masse moléculaire calculée: 737 aa, 83 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC024201
• Symbole du gène: PROX1
• Identification du gène (NCBI): 5629
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prospero-related homeobox 1 (Prox1) is a homeobox transcription factor involved in various developmental processes, including neurogenesis and lymphangiogenesis. In the central nervous system (CNS), it controls the maintenance, proliferation, and differentiation of neural stem cells. This is a rabbit polyclonal antibody raised against part of C-terminal chain of human PROX1. The calcualted molecular weight of PROX1 is 83 kDa.

What is the molecular weight of KEAP1 protein? Is Prox1 post-translationally modified?

The calculated molecular weight of Prox1 protein is 83 kDa but the observed weight is 95 kDa. Prox1 is a subject of SUMO-ylation at K556, which is important for its transcription activity and increases the molecular weight of Prox1 to 110 kDa (PMID: 19706680). Usually there is a pool of modified and unmodified Prox1 protein in cells and therefore it is quite common to observe two specific bands in western blotting using Prox1 antibody.

What is the subcellular localization of Prox1?

Prox1 contains a nuclear localization signal within its N-terminus and resides in the nucleus, where it acts as a transcription factor.

What is the tissue expression pattern of Prox1 in the central nervous system?

Prox1 expression in the central nervous system (CNS) depends on the developmental stage. During embryogenesis Prox1 is present in neuronal precursors located in the subventricular zone, while during fetal development it is additionally present in the cerebral and cerebellar cortex and hippocampus. Postnatally, Prox1 expression in the CNS is restricted to the dentate gyrus of the hippocampus and cerebellum (PMID: 17117441). Therefore, Prox1 can be used as a marker of a subset of neuronal progenitors, as well as a specific marker of dentate gyrus cell lineage, such as type-2 intermediate progenitors and mature granule cells.

Is Prox1 expressed outside of the central nervous system?

Yes. Prox1 is expressed in the developmental stages of the eye lens, liver, pancreas, heart, and the lymphatic system (PMID: 22733308). Prox1 is also expressed post-developmentally in lymphatic endothelial cells and is often used, together with VEGFR-3, as a marker for lymphatic vessels.

Publications

Species	Application	Title
mouse	IF	Nature; Meningeal lymphatic vessels at the skull base drain cerebrospinal fluid.; Authors - Ji Hoon Ahn
mouse	chIP	Nature; The role of fatty acid β-oxidation in lymphangiogenesis.; Authors - Brian W Wong
human	WB,IF	Cell Metab; Neurotensin is an anti-thermogenic peptide produced by lymphatic endothelial cells.; Authors - Jin Li
human	WB,IF	Hepatology; Prospero-related homeobox 1 drives angiogenesis of hepatocellular carcinoma through selectively activating interleukin-8 expression.; Authors - Yanfeng Liu ; KD Validated
mouse	WB,IF,ChIP	Genes Dev; The Prox1-Vegfr3 feedback loop maintains the identity and the number of lymphatic endothelial cell progenitors.; Authors - R Sathish Srinivasan ; KO Validated
human	WB	Nat Commun; Lipid droplet degradation by autophagy connects mitochondria metabolism to Prox1-driven expression of lymphatic genes and lymphangiogenesis.; Authors - Odeta Meçe

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Guang (Verified Customer) (11-12-2018)

We use heat antigen retrieval , but we didn't get any result by using this antibody. If it is possible, you can send me another free sample of this type. I will test it by using the enzymatic antigen retrieval or EDTA method to perform the Immunofluorescence.

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: mouse embryo

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