Anticorps Polyclonal de lapin anti-PRPF19

PRPF19 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 15414-1-AP

Synonymes

EC:2.3.2.27, hPso4, Pre-mRNA-processing factor 19, PRP19, PSO4

---

Galerie de données de validation

• 

  WB analysis of mouse kidney using 15414-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 15414-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15414-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15414-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15414-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15414-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15414-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach using 15414-1-AP

  Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15414-1-AP (PRPF19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules HEK-293, cellules HeLa, cellules PC-3, tissu hépatique de souris, tissu rénal de rat, tissu testiculaire humain
• Résultats positifs en IHC: tissu d'estomac humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

15414-1-AP cible PRPF19 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPF19 Protéine recombinante Ag7700
• Nom complet: PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55 kDa
• Numéro d’acquisition GenBank: BC008719
• Symbole du gène: PRPF19
• Identification du gène (NCBI): 27339
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.

Publications

Species	Application	Title
mouse	WB	Expert Opin Ther Targets; Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice.; Authors - Pei Fan
human	WB	Arch Biochem Biophys; RNF113A as a Poor Prognostic Factor Promotes Metastasis and Invasion of Cervical Cancer through miR197/PRP19/P38MAPK Signaling Pathway; Authors - Qingwei Zhang
human	WB	J Cell Biochem; Human RNF113A participates of pre-mRNA splicing in vitro.; Authors - Guilherme H Gatti da Silva
human	WB	iScience; RRM2 promotes liver metastasis of pancreatic cancer by stabilizing YBX1 and activating the TGF-beta pathway; Authors - Zhouyuan Du ; KD Validated
mouse	WB	iScience; Adipocyte-specific Steap4 deficiency reduced thermogenesis and energy expenditure in mice; Authors - Han Wang
pig,human	WB	J Virol; PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein; Authors - Xueying Zhai

{{ptg:RelatedPrimaryAntibodies}}