Anticorps Polyclonal de lapin anti-PRPH2

PRPH2 Polyclonal Antibody for WB, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugaison

Non conjugué

Publications(20)

Avis (1)

N° de cat : 18109-1-AP

Synonymes

PRPH, Peripherin-2, Peripherin2, Peripherin 2, AVMD

---

Galerie de données de validation

• 

  WB analysis of mouse eye using 18109-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 18109-1-AP (PRPH2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of mouse eye using 18109-1-AP

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (18109-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu oculaire de souris,
• Résultats positifs en IF-P: tissu oculaire de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunofluorescence (IF)-P: IF-P : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 5 publications below
• IF: See 11 publications below
• IP: See 1 publications below

Informations sur le produit

18109-1-AP cible PRPH2 dans les applications de WB, IHC, IF-P, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, poisson-zèbre, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRPH2 Protéine recombinante Ag12555
• Nom complet: peripherin 2 (retinal degeneration, slow)
• Masse moléculaire calculée: 346 aa, 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC074720
• Symbole du gène: PRPH2
• Identification du gène (NCBI): 5961
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).

Publications

Species	Application	Title
		Biomaterials; Hydrogel-mediated co-transplantation of retinal pigmented epithelium and photoreceptors restores vision in an animal model of advanced retinal degeneration.; Authors - Nikolaos Mitrousis
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	IHC	Proc Natl Acad Sci U S A; Reversal of end-stage retinal degeneration and restoration of visual function by photoreceptor transplantation.; Authors - Singh Mandeep S MS
human	IHC	Proc Natl Acad Sci U S A; Transplantation of human embryonic stem cell-derived retinal tissue in two primate models of retinal degeneration.; Authors - Hiroshi Shirai
mouse	IF	Cell Death Dis; Prph2 knock-in mice recapitulate human central areolar choroidal dystrophy retinal degeneration and exhibit aberrant synaptic remodeling and microglial activation; Authors - María José Ruiz-Pastor
mouse	IHC	Hum Mol Genet; BBS mutations modify phenotypic expression of CEP290-related ciliopathies.; Authors - Zhang Yan Y

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Melina (Verified Customer) (05-13-2024)

Worked very well in western blots of whole retina lysate

• Applications: Western Blot
• Primary Antibody Dilution: 1 ug/ml
• Cell Tissue Type: retina

PRPH2 Antibody Western Blot validation (1 ug/ml dilution) in retina (Cat no:18109-1-AP)

{{ptg:RelatedPrimaryAntibodies}}