Anticorps Polyclonal de lapin anti-PSCA

PSCA Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 17171-1-AP

Synonymes

PRO232, prostate stem cell antigen, PSCA

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Galerie de données de validation

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  WB analysis of PC-3 using 17171-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 17171-1-AP (PSCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17171-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17171-1-AP (PSCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, cellules PC-3, tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below

Informations sur le produit

17171-1-AP cible PSCA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSCA Protéine recombinante Ag10710
• Nom complet: prostate stem cell antigen
• Masse moléculaire calculée: 123 aa, 13 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC023582
• Symbole du gène: PSCA
• Identification du gène (NCBI): 8000
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prostate stem cell antigen (PSCA) is a protein composed of 123 amino acid residues. PSCA belongs to the LY-6/Thy-1 family of cell surface antigens. It is highly expressed in normal prostate and further up-regulated in prostate cancer, as well as non-prostatic malignancies including gastric cancer. PSCA plays a critical role in cell adhesion, proliferation, and survival. The obasrved MW of PSCA is between 36 to 40 kDa in paper (PMID: 11309275).

Publications

Species	Application	Title
mouse	WB	Endocrinology; mTOR regulates mineralocorticoid receptor transcriptional activity by ULK1- dependent and independent mechanisms; Authors - Yusuf Ali
human	WB	PLoS One; Pan-cancer analysis of PSCA that is associated with immune infiltration and affects patient prognosis; Authors - Chenxing Wang ; KD Validated
mouse	WB	J Immunother Cancer; Impact of tumor localization on antitumor immunity with conditionally activated CTLA-4 blockade; Authors - Marcel Arias-Badia

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