Anticorps Polyclonal de lapin anti-PSMA3

PSMA3 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11887-1-AP

Synonymes

20S proteasome, HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3

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Galerie de données de validation

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  WB analysis of A549 using 11887-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human lung using 11887-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 11887-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11887-1-AP (PSMA3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 11887-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 11887-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 11887-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 11887-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11887-1-AP (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11887-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PSMA3 antibody (11887-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules COS-7, cellules HeLa, cellules NIH/3T3, cellules PC-12, cellules RAW 264.7, tissu cérébral de rat, tissu hépatique de souris, tissu pulmonaire humain, tissu splénique de souris
• Résultats positifs en IHC: tissu de lymphome humain, tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

11887-1-AP cible PSMA3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMA3 Protéine recombinante Ag2495
• Nom complet: proteasome (prosome, macropain) subunit, alpha type, 3
• Masse moléculaire calculée: 255 aa, 28 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC029402
• Symbole du gène: PSMA3
• Identification du gène (NCBI): 5684
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

Publications

Species	Application	Title
human	ELISA	Free Radic Biol Med; Identification of an unstable 4-hydroxynoneal modification on the 20S proteasome subunit α7 by recombinant antibody technology.; Authors - Jesper Just
mouse	IF	Clin Transl Med; FBXW24 controls female meiotic prophase progression by regulating SYCP3 ubiquitination.; Authors - Yang Wang
		Cell Mol Life Sci; The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S.; Authors - Diego Sbardella
human	WB	ChemMedChem; Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity.; Authors - Anna Maria Santoro
human	WB	Research (Wash D C); SERBP1 Promotes Stress Granule Clearance by Regulating 26S Proteasome Activity and G3BP1 Ubiquitination and Protects Male Germ Cells from Thermostimuli Damage; Authors - Fengli Wang
human	WB	iScience; Guanylate-binding protein 1 modulates proteasomal machinery in ovarian cancer; Authors - Dhanir Tailor