Anticorps Polyclonal de lapin anti-PSMA4

• Phare
• Validé par KD/KO

PSMA4 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

Avis (2)

N° de cat : 11943-2-AP

Synonymes

HC9, Proteasome subunit alpha type-4, PSC9

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Galerie de données de validation

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  WB analysis using 11943-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11943-2-AP

  WB result of PSMA4 antibody (11943-2-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA4 transfected HeLa cells.

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  WB analysis of mouse kidney using 11943-2-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 11943-2-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 11943-2-AP (PSMA4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of BxPC-3 using 11943-2-AP

  IP result of anti-PSMA4 (IP:11943-2-AP, 4ug; Detection:11943-2-AP 1:500) with BxPC-3 cells lysate 2800ug.

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  IHC staining of mouse liver using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human pancreas cancer using 11943-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11943-2-AP (PSMA4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 11943-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11943-2-AP (PSMA4 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules K-562, cellules U-937, tissu rénal de souris, tissu splénique de souris
• Résultats positifs en IP: cellules BxPC-3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu hépatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

11943-2-AP cible PSMA4 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMA4 Protéine recombinante Ag2542
• Nom complet: proteasome (prosome, macropain) subunit, alpha type, 4
• Masse moléculaire calculée: 237 aa, 27 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC056249
• Symbole du gène: PSMA4
• Identification du gène (NCBI): 5685
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IHC	J Biol Chem; PITHD1 is a proteasome-interacting protein essential for male fertilization.; Authors - Hiroyuki Kondo
mouse	IHC	Sci Rep; Proteasome inhibition by bortezomib parallels a reduction in head and neck cancer cells growth, and an increase in tumor-infiltrating immune cells.; Authors - Monica Benvenuto
human	WB	PLoS One; Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.; Authors - Jayashree Chadchankar
human	WB	Cell Death Differ; Quantitative reactive cysteinome profiling reveals a functional link between ferroptosis and proteasome-mediated degradation; Authors - Yankun Wang
human	WB	Cell Death Dis; Dihydrolipoamide dehydrogenase (DLD) is a novel molecular target of bortezomib; Authors - Yu Feng

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-29-2025)

Perfect antibody! Detection in human RKO cells

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: RKO

PSMA4 Antibody Western Blot validation (1:3000 dilution) in RKO (Cat no:11943-2-AP)

FH

Tsimafei (Verified Customer) (08-12-2024)

Excellent antibody. The membrane was incubated with primary antibody at 4C, ON

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mESCs

PSMA4 Antibody Western Blot validation (1:2000 dilution) in mESCs (Cat no:11943-2-AP)

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