Anticorps Polyclonal de lapin anti-PSMA6

PSMA6 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 11573-1-AP

Synonymes

20S proteasome, 27 kDa prosomal protein, IOTA, Macropain iota chain, p27K, PROS 27, PROS27, Proteasome iota chain, PSMA6

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Galerie de données de validation

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  WB analysis using 11573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11573-1-AP (PSMA6 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 11573-1-AP

  IP Result of anti-PSMA6 (IP:11573-1-AP, 4ug; Detection:11573-1-AP 1:1000) with HEK-293 cells lysate 3200ug.

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  IHC staining of human lung cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of rat small intestine using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse colon using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11573-1-AP (PSMA6 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 11573-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 11573-1-AP (PSMA6 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11573-1-AP

  Immunofluorescent analysis of HeLa cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of MCF-7 using 11573-1-AP

  Immunofluorescent analysis of MCF-7 cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 11573-1-AP

  Immunofluorescent analysis of HepG2 cells using 11573-1-AP (PSMA6 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of HepG2 using 11573-1-AP

  1X10^6 HepG2 cells were intracellularly stained with 0.2 ug Anti-Human PSMA6 (11573-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules Neuro-2a
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu de cancer de la thyroïde humain, tissu de côlon de souris, tissu d'intestin grêle de rat, tissu testiculaire de rat, tissu testiculaire de souris, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa, cellules HepG2, cellules MCF-7
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

11573-1-AP cible PSMA6 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, oyster
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMA6 Protéine recombinante Ag2154
• Nom complet: proteasome (prosome, macropain) subunit, alpha type, 6
• Masse moléculaire calculée: 246 aa, 27 kDa
• Poids moléculaire observé: 27-29 kDa
• Numéro d’acquisition GenBank: BC023659
• Symbole du gène: PSMA6
• Identification du gène (NCBI): 5687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMA6(Proteasome subunit alpha type-6) is also named as PROS27 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It also has an ATP-dependent proteolytic activity.

Publications

Species	Application	Title
human	WB,IF	Small; Inherent Capability of Self-Assembling Nanostructures in Specific Proteasome Activation for Cancer Cell Pyroptosis; Authors - Qian-Wei Luo
oyster	WB,IF	Proteomics; A comparative proteomic analysis reveals important proteins for the fertilization and early embryonic development of the oyster Crassostrea gigas.; Authors - Xiaohong Huang

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C