Anticorps Polyclonal de lapin anti-PSMB2

• Phare
• Validé par KD/KO

PSMB2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15154-1-AP

Synonymes

20S proteasome, HC7 I, Macropain subunit C7 I, Proteasome component C7 I, Proteasome subunit beta type 2, PSMB2

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Galerie de données de validation

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  WB analysis using 15154-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15154-1-AP

  WB result of PSMB2 antibody (15154-1-AP; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB2 transfected HeLa cells.

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  WB analysis of HeLa using 15154-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 15154-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15154-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 15154-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15154-1-AP (PSMB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 15154-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 15154-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 15154-1-AP

  Immunofluorescent analysis of HepG2 cells using 15154-1-AP (PSMB2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HEK-293, cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below

Informations sur le produit

15154-1-AP cible PSMB2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMB2 Protéine recombinante Ag7198
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 2
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 23 kDa
• Numéro d’acquisition GenBank: BC000268
• Symbole du gène: PSMB2
• Identification du gène (NCBI): 5690
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB2, Proteasome subunit beta type-2, is also known as 20S proteasome subunit beta-4(PMID: 7918633). The proteasome is responsible for degradation of short lived and misfolded cytosolic and nuclear proteins in the cell and cleaves peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMB2 is up-regulated in ovarian cancer cell lines. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD (vitamin A depletion) brain regions (PMID: 21190828). Knockdown of PSMB2 suppresses hepatocellular carcinoma and gastric cancer cell proliferation and invasion (PMID: 29780166, PMID: 36110152).

Publications

Species	Application	Title
human	WB	Oncogene; NUDT21 negatively regulates PSMB2 and CXXC5 by alternative polyadenylation and contributes to hepatocellular carcinoma suppression.; Authors - Sheng Tan ; KD Validated
human	WB	Mol Cancer Res; Proteasome subunits differentially control Myeloma cell viability and proteasome inhibitor sensitivity.; Authors - Chang-Xin Shi
mouse	WB	Front Pharmacol; Selective Inhibition of the Immunoproteasome β5i Prevents PTEN Degradation and Attenuates Cardiac Hypertrophy.; Authors - Xin Xie
rat	WB	Metab Brain Dis; Molecular alteration of the proteasome contributes to AD-like pathology in the brain of HFD-STZ diabetic rats; Authors - Han Gao
rat	WB	Cell Mol Neurobiol; Inhibition of the Proteasome Regulator PA28 Aggravates Oxidized Protein Overload in the Diabetic Rat Brain; Authors - Dong-Gui Wu