Anticorps Polyclonal de lapin anti-PSMB6

PSMB6 Polyclonal Antibody for IF, IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 11684-2-AP

Synonymes

20S proteasome, DELTA, LMPY, Macropain delta chain, Proteasome delta chain, Proteasome subunit beta type 6, Proteasome subunit Y, PSMB6

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Galerie de données de validation

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  WB analysis using 11684-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 11684-2-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11684-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human liver using 11684-2-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 11684-2-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of Caco-2 using 11684-2-AP

  Caco-2 cells were subjected to SDS PAGE followed by western blot with 11684-2-AP (PSMB6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11684-2-AP (PSMB6 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11684-2-AP (PSMB6 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 11684-2-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas using 11684-2-AP (PSMB6 antibody) at dilution of 1:50 (under 25x lens).

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  IF Staining of HeLa using 11684-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11684-2-AP (PSMB6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Caco-2, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain fœtal, tissu hépatique humain
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

11684-2-AP cible PSMB6 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMB6 Protéine recombinante Ag2296
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 6
• Masse moléculaire calculée: 239 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC000835
• Symbole du gène: PSMB6
• Identification du gène (NCBI): 5694
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB6(Proteasome subunit beta type-6) is also named as LMPY, Y and belongs to the peptidase T1B family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It may also catalyzes basal processing of intracellular antigens.It can be up-regulated in anaplastic thyroid cancer cell lines and down-regulated by IFNG/IFN-gamma (at protein level)(PMID:8066462;15613457).

Publications

Species	Application	Title
rat	WB	Cell Physiol Biochem; The Favored Mechanism for Coping with Acute Cold Stress: Upregulation of miR-210 in Rats.; Authors - Wenjin Guo
mouse	WB	J Biol Chem; Hepatocyte-specific Wtap deficiency promotes hepatocellular carcinoma by activating GRB2/ERK depending on downregulation of proteasome-related genes; Authors - Xinzhi Li
mouse	WB	Biochem Pharmacol; Melatonin inhibits angiotensin II-induced atrial fibrillation through preventing degradation of Ang II Type I Receptor-Associated Protein (ATRAP); Authors - Xin Xie
mouse	WB	JHEP Rep; Downregulation of hepatic METTL3 contributes to APAP-induced liver injury in mice; Authors - Chunhong Liu