Anticorps Polyclonal de lapin anti-PSMB9

• Phare
• Validé par KD/KO

PSMB9 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 14544-1-AP

Synonymes

20S proteasome, beta1i, LMP2, Low molecular mass protein 2, Macropain chain 7, Proteasome chain 7, Proteasome subunit beta 1i, Proteasome subunit beta type 9, PSMB6i, PSMB9, RING12

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Galerie de données de validation

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  WB analysis using 14544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14544-1-AP (PSMB9 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 14544-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 14544-1-AP (LMP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 14544-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 14544-1-AP (LMP2 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14544-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14544-1-AP (PSMB9 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of Raji using 14544-1-AP

  IP Result of anti-LMP2 (IP:14544-1-AP, 4ug; Detection:14544-1-AP 1:300) with Raji cells lysate 2800ug.

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  IHC staining of human spleen using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14544-1-AP (PSMB9 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:25 (under 10x lens).

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  IHC staining of human tonsillitis using 14544-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis using 14544-1-AP (LMP2 antibody) at dilution of 1:25 (under 40x lens).

• 

  IF Staining of HepG2 using 14544-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 14544-1-AP (LMP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HL-60, cellules Raji, tissu hépatique de rat, tissu hépatique de souris, tissu pulmonaire de souris, tissu rénal de souris, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules Raji
• Résultats positifs en IHC: tissu splénique humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Informations sur le produit

14544-1-AP cible PSMB9 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMB9 Protéine recombinante Ag6048
• Nom complet: proteasome (prosome, macropain) subunit, beta type, 9 (large multifunctional peptidase 2)
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 20-23 kDa
• Numéro d’acquisition GenBank: BC065513
• Symbole du gène: PSMB9
• Identification du gène (NCBI): 5698
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMB9, also named as LMP2, PSMB6i and RING12, belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. Replacement of PSMB6 by PSMB9 increases the capacity of the immunoproteasome to cleave model peptides after hydrophobic and basic residues. High expression of PSMB9 associated with the poor prognosis of patients with NPC.

Publications

Species	Application	Title
human	WB	Mol Cell; DNA damage promotes HLA class I presentation by stimulating a pioneer round of translation-associated antigen production.; Authors - Yuki Uchihara ; KD Validated
human	IF	Cell Res; In-cell infection: a novel pathway for Epstein-Barr virus infection mediated by cell-in-cell structures.; Authors - Chao Ni
mouse		EMBO J; Proteasomal degradation within endocytic organelles mediates antigen cross-presentation.; Authors - Debrup Sengupta
human	WB	Cancer Lett; Deciphering the tumor-suppressive role of PSMB9 in melanoma through multi-omics and single-cell transcriptome analyses; Authors - Xing Hu
mouse	WB	Cell Rep; The portrait of liver cancer is shaped by mitochondrial genetics.; Authors - Mrittika Chattopadhyay
human	IF,WB	Front Oncol; Immune Characteristics and Prognosis Analysis of the Proteasome 20S Subunit Beta 9 in Lower-Grade Gliomas.; Authors - Junzhe Liu