Anticorps Polyclonal de lapin anti-PSMC1

PSMC1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 11196-1-AP

Synonymes

P26S4, p56, PSMC1, S4

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Galerie de données de validation

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  WB analysis of mouse brain using 11196-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11196-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 11196-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 11196-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11196-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11196-1-AP (PSMC1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules HeLa, cellules Jurkat, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

11196-1-AP cible PSMC1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMC1 Protéine recombinante Ag1677
• Nom complet: proteasome (prosome, macropain) 26S subunit, ATPase, 1
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 52-57 kDa
• Numéro d’acquisition GenBank: BC016368
• Symbole du gène: PSMC1
• Identification du gène (NCBI): 5700
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMC1(26S protease regulatory subunit 4) is also named as p26s4 and belongs to the AAA ATPase family.The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex.

Publications

Species	Application	Title
human,mouse	WB	Sci Adv; Lipid-anchored proteasomes control membrane protein homeostasis; Authors - Ruizhu Zhang
human	WB	Oncogene; The degradation of p53 and its major E3 ligase Mdm2 is differentially dependent on the proteasomal ubiquitin receptor S5a.; Authors - Sparks A A
		Cell Mol Life Sci; The insulin-degrading enzyme is an allosteric modulator of the 20S proteasome and a potential competitor of the 19S.; Authors - Diego Sbardella
human	WB	PLoS One; Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells.; Authors - Jayashree Chadchankar
rat	WB	Neuropathology; Adenoviral expression of TDP-43 and FUS genes and shRNAs for protein degradation pathways in rodent motoneurons in vitro and in vivo.; Authors - Watabe Kazuhiko K