Anticorps Polyclonal de lapin anti-PSMC2

• Phare
• Validé par KD/KO

PSMC2 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 14905-1-AP

Synonymes

MSS1, Nbla10058, Protein MSS1, PSMC2, S7

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Galerie de données de validation

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  WB analysis using 14905-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 14905-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14905-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 14905-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 14905-1-AP (PSMC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 14905-1-AP

  IP Result of anti-PSMC2 (IP:14905-1-AP, 4ug; Detection:14905-1-AP 1:500) with mouse spleen tissue lysate 4800ug.

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  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 14905-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14905-1-AP (PSMC2 Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu de glande surrénale humain, tissu splénique de souris
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 4 publications below

Informations sur le produit

14905-1-AP cible PSMC2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMC2 Protéine recombinante Ag6700
• Nom complet: proteasome (prosome, macropain) 26S subunit, ATPase, 2
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC002589
• Symbole du gène: PSMC2
• Identification du gène (NCBI): 5701
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMC2 is also named as MSS1 and belongs to the AAA ATPase family. PSMC2 is 1 of 6 putative ATPases contained within the regulatory complex. PSMC2 is first identified as a possible cellular factor that cooperates with the human immunodeficiency virus-1 (HIV-1) protein Tat, a potent activator of virus gene expression. The PSMC2 gene product works in association with the small subunit of mitoribosomes and seems to play some part in mitochondrial translation(PMID:8392589).

Publications

Species	Application	Title
human	IHC	Gastroenterology; Proteomic characterization identifies clinically relevant subgroups of gastrointestinal stromal tumors; Authors - Mingjun Sun
human	WB	Front Immunol; PDLIM7 Synergizes With PDLIM2 and p62/Sqstm1 to Inhibit Inflammatory Signaling by Promoting Degradation of the p65 Subunit of NF-κB.; Authors - Aya Jodo
rat	WB	Cardiovasc Drugs Ther; Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.; Authors - Kuixiang Chen
mouse	IHC,WB	Calcif Tissue Int; Silencing Proteasome 26S Subunit ATPase 2 (PSMC2) Protects the Osteogenic Differentiation In Vitro and Osteogenesis In Vivo.; Authors - Bin Zhou ; KD Validated
human	IHC	Oncol Lett; PSMC2, ORC5 and KRTDAP are specific biomarkers for HPV-negative head and neck squamous cell carcinoma.; Authors - Yushen Su
human	IHC	Pharmgenomics Pers Med; PSMC2 is a Novel Prognostic Biomarker and Predicts Immunotherapeutic Responses: From Pancreatic Cancer to Pan-Cancer; Authors - Wei Huang