Anticorps Polyclonal de lapin anti-PSMC3

PSMC3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 24142-1-AP

Synonymes

TBP-1, TBP1, Tat-binding protein 1, 26S proteasome regulatory subunit 6A, 26S proteasome AAA-ATPase subunit RPT5

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Galerie de données de validation

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  WB analysis using 24142-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 24142-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 24142-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 24142-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24142-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 24142-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 24142-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 24142-1-AP (PSMC3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 24142-1-AP

  IP result of anti-PSMC3 (IP:24142-1-AP, 4ug; Detection:24142-1-AP 1:800) with HeLa cells lysate 3200ug.

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  IHC staining of human ovary tumor using 24142-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 24142-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human tonsillitis using 24142-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human tonsillitis using 24142-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 24142-1-AP (PSMC3 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HEK-293 using 24142-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using PSMC3 antibody (24142-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, cellules HepG2, cellules Jurkat, cellules MCF-7, cellules T-47D
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu d'amygdalite humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HEK-293,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below

Informations sur le produit

24142-1-AP cible PSMC3 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMC3 Protéine recombinante Ag21408
• Nom complet: proteasome (prosome, macropain) 26S subunit, ATPase, 3
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC008713
• Symbole du gène: PSMC3
• Identification du gène (NCBI): 5702
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMC3(26S protease regulatory subunit 6A) is also named as TBP1, Proteasome 26S subunit ATPase 3 and belongs to the AAA ATPase family. The 26S protease is involved in the ATP-dependent degradation of ubiquitinated proteins. It is a multifunctional protein, component of the regulatory subunit of the proteasome, involved in different cellular processes.

Publications

Species	Application	Title
human	WB	ChemMedChem; Pyrazolones Activate the Proteasome by Gating Mechanisms and Protect Neuronal Cells from β-Amyloid Toxicity.; Authors - Anna Maria Santoro
human	WB	Mol Biol Cell; Distinct proteostasis states drive pharmacologic chaperone susceptibility for cystic fibrosis transmembrane conductance regulator misfolding mutants; Authors - Eli Fritz McDonald
human	WB	Hum Mol Genet; PSMC5 insufficiency and P320R mutation impair proteasome function; Authors - Zhong-Qiu Yu
human,mouse	WB	Elife; A cleaved METTL3 potentiates the METTL3-WTAP interaction and breast cancer progression; Authors - Chaojun Yan

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