Anticorps Polyclonal de lapin anti-PSMC6

PSMC6 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 15839-1-AP

Synonymes

CADP44, p42, P44, Proteasome subunit p42, PSMC6, Rpt4, SUG2

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Galerie de données de validation

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  WB analysis using 15839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 15839-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human testis using 15839-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15839-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15839-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15839-1-AP (PSMC6 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15839-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15839-1-AP (PSMC6 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain, tissu hépatique de rat, tissu hépatique de souris, tissu testiculaire humain
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

15839-1-AP cible PSMC6 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMC6 Protéine recombinante Ag8589
• Nom complet: proteasome (prosome, macropain) 26S subunit, ATPase, 6
• Masse moléculaire calculée: 389 aa, 44 kDa
• Poids moléculaire observé: 42 kDa
• Numéro d’acquisition GenBank: BC005390
• Symbole du gène: PSMC6
• Identification du gène (NCBI): 5706
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSMC6(proteasome (prosome, macropain) 26S subunit, ATPase, 6) is also named as CADP44, MGC12520, P44, SUG2, p42 and belongs to the AAA ATPase family. It is a component of the 26S protease, which is involved in the ATP-dependent degradation of ubiquitinated proteins. The regulatory (or ATPase) complex confers ATP dependency and substrate specificity to the 26S complex. The gene encodes a 44 kDa PSMC6.

Publications

Species	Application	Title
mouse	WB	J Biomed Sci; Znf179 E3 ligase-mediated TDP-43 polyubiquitination is involved in TDP-43- ubiquitinated inclusions (UBI) (+)-related neurodegenerative pathology.; Authors - Yi-Chao Lee
mouse	IHC	FASEB J; Glucose-induced decrease of cystathionine β-synthase mediates renal injuries.; Authors - Yanting Yu
rat	WB	Cardiovasc Drugs Ther; Quercetin Prevents In Vivo and In Vitro Myocardial Hypertrophy Through the Proteasome-GSK-3 Pathway.; Authors - Kuixiang Chen
mouse,rat	WB	Acta Pharmacol Sin; Zonisamide attenuates pressure overload-induced myocardial hypertrophy in mice through proteasome inhibition; Authors - Qian Wu
		Cell Rep Med; Integrative proteomic profiling of tumor and plasma extracellular vesicles identifies a diagnostic biomarker panel for colorectal cancer; Authors - Jun Wang

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