Anticorps Monoclonal anti-PSMD13

PSMD13 Monoclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, poulet, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2G10G6

N° de cat : 67807-1-Ig

Synonymes

HSPC027, p40.5, PSMD13, Rpn9, S11

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Galerie de données de validation

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  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of SMMC-7721 using 67807-1-Ig

  SMMC-7721 cells were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of HuH-7 using 67807-1-Ig

  HuH-7 cells were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 67807-1-Ig

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67807-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67807-1-Ig (PSMD13 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67807-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67807-1-Ig (PSMD13 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 67807-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSMD13 antibody (67807-1-Ig, Clone: 2G10G6 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2, cellules HSC-T6, cellules HuH-7, cellules Jurkat, cellules K-562, cellules L02, cellules NIH/3T3, cellules SMMC-7721, tissu hépatique de lapin, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000
• Immunofluorescence (IF): IF : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67807-1-Ig cible PSMD13 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, poulet, rat, souris

• Réactivité: Humain, Lapin, porc, poulet, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PSMD13 Protéine recombinante Ag7857
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 13
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001100
• Symbole du gène: PSMD13
• Identification du gène (NCBI): 5719
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.