Anticorps Polyclonal de lapin anti-PSMD7

• Phare
• Validé par KD/KO

PSMD7 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 16034-1-AP

Synonymes

MOV34, Mov34 protein homolog, MOV34L, P40, Proteasome subunit p40, PSMD7, Rpn8, S12

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Galerie de données de validation

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  WB analysis using 16034-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16034-1-AP (PSMD7 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16034-1-AP

  WB result of PSMD7 antibody (16034-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMD7 transfected HeLa cells.

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  IP experiment of K-562 using 16034-1-AP

  IP Result of anti-PSMD7 (IP:16034-1-AP, 3ug; Detection:16034-1-AP 1:500) with K-562 cells lysate 2400ug.

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  IHC staining of human colon cancer using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse liver using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 16034-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16034-1-AP (PSMD7 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 16034-1-AP

  Immunofluorescent analysis of HepG2 cells, using PSMD7 antibody 16034-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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  FC experiment of HepG2 using 16034-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PSMD7 antibody (16034-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules K-562, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du poumon humain, tissu hépatique de souris, tissu rénal de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below
• CoIP: See 2 publications below

Informations sur le produit

16034-1-AP cible PSMD7 dans les applications de WB, IP, IHC, IF, FC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSMD7 Protéine recombinante Ag8905
• Nom complet: proteasome (prosome, macropain) 26S subunit, non-ATPase, 7
• Masse moléculaire calculée: 324 aa, 37 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC012606
• Symbole du gène: PSMD7
• Identification du gène (NCBI): 5713
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proteasome 26S subunit, non-ATPase 7 (PSMD7), an ATP-independent component of the 19S regulatory subunit, is a member of the JAMM/MPN domain-associated metallopeptidase (JAMM) DUB family. PSMD7, as a core component of the 26S proteasome, is critical for the degradation of ubiquitinated proteins in the proteasome. PSMD7 arrests cell cycle in the G2/M phase during HIV infection. The molecular weight of PSMD7 is 37 kDa. (PMID: 34512150, 34234864)

Publications

Species	Application	Title
human	WB,CoIP	Int J Biol Sci; Deubiquitinase PSMD7 promotes the proliferation, invasion, and cisplatin resistance of gastric cancer cells by stabilizing RAD23B.; Authors - Jianjiang Wang ; KD Validated
human	WB,IF,CoIP	Cell Signal; Deubiquitinating enzyme PSMD7 promotes bladder cancer development: Involvement of RAB1A stabilization; Authors - Jun Wang