Anticorps Polyclonal de lapin anti-PSPC1

• Phare
• Validé par KD/KO

PSPC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, RIP, ELISA

Conjugaison

Non conjugué

Publications(10)

N° de cat : 16714-1-AP

Synonymes

PSP1, PSPC 1

---

Galerie de données de validation

• 

  WB analysis using 16714-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16714-1-AP (PSPC1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 16714-1-AP

  IP result of anti-PSPC1 (IP:16714-1-AP, 4ug; Detection:16714-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

• 

  IHC staining of human placenta using 16714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16714-1-AP (PSPC1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 16714-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 16714-1-AP (PSPC1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of PC-3 using 16714-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed PC-3 cells using 16714-1-AP (PSPC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HepG2, cellules PC-3
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu placentaire humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules PC-3

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• RIP: See 1 publications below

Informations sur le produit

16714-1-AP cible PSPC1 dans les applications de WB, IHC, IF/ICC, IP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSPC1 Protéine recombinante Ag10136
• Nom complet: paraspeckle component 1
• Masse moléculaire calculée: 523 aa, 59 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC014184
• Symbole du gène: PSPC1
• Identification du gène (NCBI): 55269
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSPC1 (paraspeckle component 1), a known paraspeckle biomarker, is a putative transcription factor that belongs to the Drosophila behavior/human splicing (DBHS) family. PSPC1 was recently identified as a contextual determinant of tumor progression in multiple cancer types involving oncogenic reprogramming to switch proapoptotic TGF-β to prometastatic TGF-β via hijacking of Smad2/3 targeting. Upregulated PSPC1 is correlated with advanced tumor stages and poor survival of patients with breast, lung, and liver cancers. Upregulated PSPC1 potentiates expression of mesenchymal markers, EMT transcription factors (EMT-TF), cancer stem-like cell transcription factors (CSC-TF), and c-Myc-related proliferation genes, thus promoting migration, invasion, spheroid formation, tumor formation, and metastasis.

Publications

Species	Application	Title
human	WB	Drug Resist Updat; Cell membrane-camouflaged bufalin targets NOD2 and overcomes multidrug resistance in pancreatic cancer; Authors - Wei Zhang
human	WB	Nat Commun; K235 acetylation couples with PSPC1 to regulate the m6A demethylation activity of ALKBH5 and tumorigenesis; Authors - Xiao-Lan Zhang
human	WB	Nucleic Acids Res; mRNAs are sorted for export or degradation before passing through nuclear speckles.; Authors - Jing Fan
human	WB,IF	Cancer Lett; SKP2 promotes the metastasis of pancreatic ductal adenocarcinoma by suppressing TRIM21-mediated PSPC1 degradation; Authors - Jiahui Yuan
mouse	WB	Nat Commun; Capture of RNA-binding proteins across mouse tissues using HARD-AP; Authors - Yijia Ren
human	IF	Nat Commun; Virus-modified paraspeckle-like condensates are hubs for viral RNA processing and their formation drives genomic instability; Authors - Katherine L Harper

{{ptg:RelatedPrimaryAntibodies}}