Anticorps Polyclonal de lapin anti-PSPH

• Phare
• Validé par KD/KO

PSPH Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (1)

N° de cat : 14513-1-AP

Synonymes

L 3 phosphoserine phosphatase, phosphoserine phosphatase, PSP, PSPase, PSPH

---

Galerie de données de validation

• 

  WB analysis using 14513-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14513-1-AP (PSPH antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat liver using 14513-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 14513-1-AP (PSPH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HL-60 using 14513-1-AP

  IP Result of anti-PSPH (IP:14513-1-AP, 4ug; Detection:14513-1-AP 1:300) with HL-60 cells lysate 3040ug.

• 

  IHC staining of human spleen using 14513-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14513-1-AP (PSPH Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human spleen using 14513-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 14513-1-AP (PSPH Antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 14513-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSPH antibody (14513-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules A375, cellules HL-60, cellules MCF-7, cellules MG U-87, tissu hépatique de rat
• Résultats positifs en IP: cellules HL-60
• Résultats positifs en IHC: tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 24 publications below
• IHC: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

14513-1-AP cible PSPH dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PSPH Protéine recombinante Ag5972
• Nom complet: phosphoserine phosphatase
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC063614
• Symbole du gène: PSPH
• Identification du gène (NCBI): 5723
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PSPH (phosphoserine phosphatase) is an enzyme, which is involved in the process of L-serine biosynthesis. PSPH mainly plays role in multiple aspects of cell behaviours such as proliferation and differentiation by producing precursors for the biosynthesis of diverse compounds including neurotransmitters, glycolipids and thymidine (PMID: 11237721, PMID: 19963421). Additionally, augmented PSPH level is correlated with the prognosis in multiple cancers including cutaneous squamous cell carcinoma (PMID: 21726982), breast cancer (PMID: 28931725), non-small cell lung cancer (PMID: 30662358), colorectal cancer (PMID: 24146633) and hepatocellular carcinoma (PMID: 25793315).

Publications

Species	Application	Title
		Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cell Metab; Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.; Authors - Liang Wei Wang
human	WB	Cell Metab; Serine metabolism antagonizes antiviral innate immunity by preventing ATP6V0d2-mediated YAP lysosomal degradation.; Authors - Long Shen
human	WB,IP	Nat Commun; PHGDH arginine methylation by PRMT1 promotes serine synthesis and represents a therapeutic vulnerability in hepatocellular carcinoma; Authors - Kui Wang
human	WB	Nat Commun; Phosphoglycerate dehydrogenase activates PKM2 to phosphorylate histone H3T11 and attenuate cellular senescence; Authors - Yinsheng Wu
human	WB	Mol Cell; The long noncoding RNA glycoLINC assembles a lower glycolytic metabolon to promote glycolysis.; Authors - Youming Zhu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Hua (Verified Customer) (11-30-2022)

Good antibody for WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HepG2 cells, mouse liver, and mouse brain

PSPH Antibody Western Blot validation (1:1000 dilution) in HepG2 cells, mouse liver, and mouse brain (Cat no:14513-1-AP)