Anticorps Polyclonal de lapin anti-PTBP2

• Phare
• Validé par KD/KO

PTBP2 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 55186-1-AP

Synonymes

PTB like protein, PTB, Polypyrimidine tract-binding protein 2, Neurally-enriched homolog of PTB, Neural polypyrimidine tract-binding protein

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Galerie de données de validation

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  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 55186-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 55186-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 55186-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55186-1-AP (PTBP2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse brain using 55186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 55186-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 55186-1-AP (PTBP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of mouse brain using 55186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 55186-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 55186-1-AP (PTBP2 antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

55186-1-AP cible PTBP2 dans les applications de WB, IHC, IF-P, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: polypyrimidine tract binding protein 2
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 57-60 kDa
• Numéro d’acquisition GenBank: NM_021190
• Symbole du gène: PTBP2
• Identification du gène (NCBI): 58155
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTBP2, also named as NPTB, PTB and PTBLP, is a RNA-binding protein which binds to intronic polypyrimidine tracts and mediates negative regulation of exons splicing. It may antagonize in a tissue-specific manner the ability of NOVA1 to activate exon selection. Beside its function in pre-mRNA splicing, PTBP2 plays also a role in the regulation of translation. Isoform 5 has a reduced affinity for RNA. (PMID:11003644) . Protein may exist in a dimeric form with a MW of 110-120 kDa (PMID: 20064465). This antibody is specific to PTBP2.

Publications

Species	Application	Title
human	WB	Cell Rep; PTBP1 and PTBP2 Repress Nonconserved Cryptic Exons.; Authors - Jonathan P Ling ; KD Validated
human,mouse	WB	Mol Cell Biol; RBM4 modulates radial migration via alternative splicing of Dab1 during cortex development.; Authors - Dhananjaya D
mouse	WB,IF,IP	Nat Commun; Cytosolic Ptbp2 modulates axon growth in motoneurons through axonal localization and translation of Hnrnpr; Authors - Saeede Salehi ; KD Validated
human	WB	Nucleic Acids Res; N-terminal domain of polypyrimidine-tract binding protein is a dynamic folding platform for adaptive RNA recognition; Authors - Fred F Damberger
mouse	WB,IF,CoIP	Front Mol Neurosci; Ptbp2 re-expression rescues axon growth defects in Smn-deficient motoneurons; Authors - Saeede Salehi
mouse	WB	Cell Rep; Interrogation of RNA-bound proteome with XRNAX illuminates molecular alterations in the mouse brain affected with dysmyelination; Authors - Marta Sztachera

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