Anticorps Polyclonal de lapin anti-PTCD3

PTCD3 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 25158-1-AP

Synonymes

PTCD3, TRG 15

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Galerie de données de validation

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  WB analysis of mouse embryo using 25158-1-AP

  mouse embryo tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 25158-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 25158-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 25158-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25158-1-AP (PTCD3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 25158-1-AP

  IP result of anti-PTCD3 (IP:25158-1-AP, 4ug; Detection:25158-1-AP 1:600) with HEK-293 cells lysate 3600ug.

• 

  IF Staining of HEK-293 using 25158-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HEK-293 cells using 25158-1-AP (PTCD3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu embryonnaire de souris, cellules HEK-293, tissu cardiaque de rat, tissu rénal de rat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• CoIP: See 1 publications below
• RIP: See 1 publications below

Informations sur le produit

25158-1-AP cible PTCD3 dans les applications de WB, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTCD3 Protéine recombinante Ag18330
• Nom complet: Pentatricopeptide repeat domain 3
• Masse moléculaire calculée: 689 aa, 79 kDa
• Poids moléculaire observé: 78-80 kDa
• Numéro d’acquisition GenBank: BC011832
• Symbole du gène: PTCD3
• Identification du gène (NCBI): 55037
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
		iScience; Supernumerary proteins of the human mitochondrial ribosomal small subunit are integral for assembly and translation; Authors - Taru Hilander
human	WB	Cell Death Dis; MRPS31 loss is a key driver of mitochondrial deregulation and hepatocellular carcinoma aggressiveness.; Authors - Seongki Min
human	WB	Biomedicines; Mitochondrial Ribosome Dysfunction in Human Alveolar Type II Cells in Emphysema; Authors - Loukmane Karim
human	WB	J Gen Virol; Inhibition of human cytomegalovirus replication by interferon alpha can involve multiple anti-viral factors; Authors - Shabab Chowdhury
human	WB	Nat Struct Mol Biol; A roadmap for ribosome assembly in human mitochondria; Authors - Elena Lavdovskaia
human	RIP,CoIP	FASEB J; Epigenetic Activation of PTCD3 Promotes CRC Glutamine Metabolism and Metastasis via IGF2BP2-Mediated SLC38A2 m6A Modification; Authors - Weihui Peng

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