Anticorps Polyclonal de lapin anti-PTGDS

PTGDS Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(11)

N° de cat : 10754-2-AP

Synonymes

LPGDS, Lipocalin-type prostaglandin-D synthase, Glutathione-independent PGD synthase, EC:5.3.99.2, Cerebrin-28

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Galerie de données de validation

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  WB analysis of HeLa using 10754-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10754-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10754-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human kidney using 10754-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 10754-2-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 10754-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 10754-2-AP

  IP result of anti-PTGDS (IP:10754-2-AP, 4ug; Detection:10754-2-AP 1:600) with mouse brain tissue lysate 1840 ug.

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  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3, plasma humain, tissu cardiaque humain, tissu de côlon humain, tissu rénal humain, tissu urinaire humain
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 6 publications below
• IHC: See 5 publications below
• IF: See 1 publications below

Informations sur le produit

10754-2-AP cible PTGDS dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTGDS Protéine recombinante Ag1163
• Nom complet: prostaglandin D2 synthase 21kDa (brain)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC005939
• Symbole du gène: PTGDS
• Identification du gène (NCBI): 5730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Glycoprotein prostaglandin D2 synthase (PTGDS), also known as L-PGDS, PGDS, and LPGDS, is a member of the lipocalin superfamily and plays dual roles in prostaglandins metabolism and lipid transport (PMID: 34743203). PTGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PTGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. PTGDS is abundantly expressed in the brain and heart and has also been found in testis, prostate, kidney, spleen and leukocytes. It can be found as a collection of monomeric glycoforms with molecular weights in the 27-34 kDa range (PMID: 32430846).

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway.; Authors - Hui-Yuan Su
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
human	WB	Cell Cycle; MicroRNA-138 improves LPS-induced trophoblast dysfunction through targeting RELA and NF-κB signaling.; Authors - Ailan Yin
human	IHC	Cancer Manag Res; Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation.; Authors - Ruoyao Zou
human	IHC	Discov Oncol; Transcriptomic analysis reveals potential crosstalk genes and immune relationship between triple-negative breast cancer and depression; Authors - Zhili Zhuo
human	WB	Med Sci Monit; Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.; Authors - Kim Jae Ho JH

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