Anticorps Polyclonal de lapin anti-PTGDS

PTGDS Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 10754-2-AP

Synonymes

Beta trace protein, Cerebrin 28, LPGDS, PDS, PGD2, PGD2 synthase, PGDS, PGDS2, Prostaglandin D2 synthase, Prostaglandin H2 D isomerase, PTGDS

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Galerie de données de validation

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  WB analysis of HeLa using 10754-2-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 10754-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of human colon using 10754-2-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 10754-2-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10754-2-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 10754-2-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10754-2-AP (PTGDS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 10754-2-AP (PTGDS antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10754-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10754-2-AP (PTGDS antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules PC-3, plasma humain, tissu cardiaque humain, tissu de côlon humain, tissu rénal humain, tissu urinaire humain
• Résultats positifs en IHC: tissu cérébral de souris, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:3000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 2 publications below

Informations sur le produit

10754-2-AP cible PTGDS dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTGDS Protéine recombinante Ag1163
• Nom complet: prostaglandin D2 synthase 21kDa (brain)
• Masse moléculaire calculée: 21 kDa
• Poids moléculaire observé: 28 kDa
• Numéro d’acquisition GenBank: BC005939
• Symbole du gène: PTGDS
• Identification du gène (NCBI): 5730
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PGDS(Prostaglandin-H2 D-isomerase) is also named as PTGDS, PDS, PGDS2. It catalyzes the conversion of PGH2 to PGD2, and is a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. It has two N-glycosylation recognition sites.

Publications

Species	Application	Title
mouse	WB	Int J Mol Sci; Zileuton, a 5-Lipoxygenase Inhibitor, Attenuates Haemolysate-Induced BV-2 Cell Activation by Suppressing the MyD88/NF-κB Pathway.; Authors - Hui-Yuan Su
pig	WB	Front Cell Dev Biol; Comparative Proteomics and Phosphoproteomics Analysis Reveal the Possible Breed Difference in Yorkshire and Duroc Boar Spermatozoa.; Authors - Yongjie Xu
human	WB	Cell Cycle; MicroRNA-138 improves LPS-induced trophoblast dysfunction through targeting RELA and NF-κB signaling.; Authors - Ailan Yin
human	IHC	Cancer Manag Res; Decreased PTGDS Expression Predicting Poor Survival of Endometrial Cancer by Integrating Weighted Gene Co-Expression Network Analysis and Immunohistochemical Validation.; Authors - Ruoyao Zou
human	WB	Med Sci Monit; Proteome analysis of human cerebrospinal fluid as a diagnostic biomarker in patients with meningioma.; Authors - Kim Jae Ho JH
mouse	IHC	Mol Neurodegener; MLKL deficiency alleviates neuroinflammation and motor deficits in the α-synuclein transgenic mouse model of Parkinson's disease; Authors - Lu Geng