Anticorps Polyclonal de lapin anti-PTGER4

PTGER4 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(15)

N° de cat : 24895-1-AP

Synonymes

EP4R, EP4

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Galerie de données de validation

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  WB analysis of rat brain using 24895-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24895-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 24895-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HL-60, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 13 publications below
• IHC: See 2 publications below
• IF: See 5 publications below

Informations sur le produit

24895-1-AP cible PTGER4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTGER4 Protéine recombinante Ag19217
• Nom complet: prostaglandin E receptor 4 (subtype EP4)
• Masse moléculaire calculée: 488 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC101534
• Symbole du gène: PTGER4
• Identification du gène (NCBI): 5734
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTGER4 (Prostaglandin E2 receptor EP4 subtype, also known as EP4R) is a member of the G-protein coupled receptor family. This protein is one of four receptors identified for prostaglandin E2 (PGE2). May play an important role in regulating renal hemodynamics, intestinal epithelial transport, adrenal aldosterone secretion, and uterine function.

Publications

Species	Application	Title
human	WB	J Clin Invest; Antibiotic pretreatment alleviates liver transplant damage in mice and humans.; Authors - Kojiro Nakamura
rat	WB	J Cell Mol Med; Inhibition of prostaglandin E2 receptor 4 by lnc000908 to promote the endothelial-mesenchymal transition participation in cardiac remodelling.; Authors - Xingxing Chen
rat	WB	Mol Pharm; PMID: 30109938; Authors - Huan Li
human	WB	Int J Mol Sci; Correlative Study on Impaired Prostaglandin E2 Regulation in Epicardial Adipose Tissue and its Role in Maladaptive Cardiac Remodeling via EPAC2 and ST2 Signaling in Overweight Cardiovascular Disease Subjects.; Authors - Elena Vianello
mouse	WB	Biomolecules; In Silico, In Vitro, and In Vivo Analysis of Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza as Modulators of Cyclooxygenase-2/mPGES-1/Endothelial Prostaglandin EP3 Pathway.; Authors - Anella Saviano
	IF	Exp Cell Res; Prostaglandin E2 accelerated recovery of chemotherapy-induced intestinal damage by increasing expression of cyclin D.; Authors - Mengzhen Yue

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