Anticorps Polyclonal de lapin anti-PTGER4

PTGER4 Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 24895-1-AP

Synonymes

EP4, EP4R, PGE receptor EP4 subtype, PGE2 receptor EP4 subtype, Prostanoid EP4 receptor, PTGER2, PTGER4

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Galerie de données de validation

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  WB analysis of rat brain using 24895-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 24895-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 24895-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 24895-1-AP (PTGER4 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human prostate hyperplasia using 24895-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 24895-1-AP (PTGER4 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de rat, cellules HL-60, tissu cérébral de souris
• Résultats positifs en IHC: tissu d'hyperplasie de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 8 publications below
• IF: See 2 publications below

Informations sur le produit

24895-1-AP cible PTGER4 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTGER4 Protéine recombinante Ag19217
• Nom complet: prostaglandin E receptor 4 (subtype EP4)
• Masse moléculaire calculée: 488 aa, 53 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC101534
• Symbole du gène: PTGER4
• Identification du gène (NCBI): 5734
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Clin Invest; Antibiotic pretreatment alleviates liver transplant damage in mice and humans.; Authors - Kojiro Nakamura
rat	WB	J Cell Mol Med; Inhibition of prostaglandin E2 receptor 4 by lnc000908 to promote the endothelial-mesenchymal transition participation in cardiac remodelling.; Authors - Xingxing Chen
rat	WB	Mol Pharm; PMID: 30109938; Authors - Huan Li
human	WB	Int J Mol Sci; Correlative Study on Impaired Prostaglandin E2 Regulation in Epicardial Adipose Tissue and its Role in Maladaptive Cardiac Remodeling via EPAC2 and ST2 Signaling in Overweight Cardiovascular Disease Subjects.; Authors - Elena Vianello
mouse	WB	Biomolecules; In Silico, In Vitro, and In Vivo Analysis of Tanshinone IIA and Cryptotanshinone from Salvia miltiorrhiza as Modulators of Cyclooxygenase-2/mPGES-1/Endothelial Prostaglandin EP3 Pathway.; Authors - Anella Saviano
	IF	Exp Cell Res; Prostaglandin E2 accelerated recovery of chemotherapy-induced intestinal damage by increasing expression of cyclin D.; Authors - Mengzhen Yue

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