Anticorps Polyclonal de lapin anti-BRK

• Phare
• Validé par KD/KO

BRK Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 18697-1-AP

Synonymes

Breast tumor kinase, BRK, Protein tyrosine kinase 6, PTK6, PTK6 protein tyrosine kinase 6, Tyrosine protein kinase BRK

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Galerie de données de validation

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  WB analysis of HeLa using 18697-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18697-1-AP (BRK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18697-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18697-1-AP (BRK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 18697-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 18697-1-AP (BRK antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spleen using 18697-1-AP

  rat spleen tissue were subjected to SDS PAGE followed by western blot with 18697-1-AP (BRK antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 18697-1-AP

  IP Result of anti-BRK (IP:18697-1-AP, 4ug; Detection:18697-1-AP 1:800) with HeLa cells lysate 3440ug.

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  IHC staining of human colon using 18697-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18697-1-AP (BRK antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human colon using 18697-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon using 18697-1-AP (BRK antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of MCF-7 using 18697-1-AP

  Immunofluorescent analysis of MCF-7 cells, using PTK6 antibody 18697-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, tissu splénique de rat, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules MCF-7

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

18697-1-AP cible BRK dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: PTK6 protein tyrosine kinase 6
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC035843
• Symbole du gène: PTK6
• Identification du gène (NCBI): 5753
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

BRK, also named as PTK6 and FLJ42088, is a cytoplasmic nonreceptor protein kinase which may function as an intracellular signal transducer in epithelial tissues. PTK6 can phosphorylate KHDRBS1, KHDRBS2, KHDRBS3 and STAP2/BKS. Overexpression of PTK6 in mammary epithelial cells leads to sensitization of the cells to epidermal growth factor and results in a partially transformed phenotype. Expression of PTK6 has been detected at low levels in some breast tumors but not in normal breast tissue. Its presence in the nucleus appears to be linked to suppression of tumor progression. This antibody is specific to PTK6.

Publications

Species	Application	Title
human	WB	Mol Cancer Res; RSK Promotes Prostate Cancer Progression in Bone through ING3, CKAP2 and PTK6-mediated Cell Survival.; Authors - Guoyu Yu ; KD Validated
human	WB	Chin J Cancer; The expression and prognostic value of protein tyrosine kinase 6 in early-stage cervical squamous cell cancer.; Authors - Xiao-Jing Wang
human	WB	Dose Response; Genome-Wide Identification of Autophagy Prognostic Signature in Pancreatic Cancer.; Authors - Jianfa Yu
human	IF	Adv Sci (Weinh); ETV4 Mediated Tumor-Associated Neutrophil Infiltration Facilitates Lymphangiogenesis and Lymphatic Metastasis of Bladder Cancer; Authors - Qiang Zhang ; KD Validated