Anticorps Polyclonal de lapin anti-PTP4A1/PRL1

• Phare
• Validé par KD/KO

PTP4A1/PRL1 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 11508-1-AP

Synonymes

HH72, PRL 1, PRL1, PTP(CAAX1), PTP(CAAXI), PTP4A1, PTP4A1/PRL1, PTPCAAX1

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Galerie de données de validation

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  WB analysis of HepG2 using 11508-1-AP

  WB result of PTP4A1/PRL1 antibody (11508-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP4A1/PRL1 transfected HepG2 cells.

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  WB analysis using 11508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11508-1-AP (PTP4A1/PRL1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 11508-1-AP

  WB result of PTP4A1/PRL1 antibody (11508-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP4A1/PRL1 transfected HeLa cells.

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  WB analysis of MCF-7 using 11508-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 11508-1-AP (PTP4A1/PRL1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas cancer using 11508-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11508-1-AP (PTP4A1/PRL1 antibody) at dilution of 1:100 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 11508-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 11508-1-AP (PTP4A1/PRL1 antibody) at dilution of 1:100 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu de cancer du pancréas humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IHC: See 2 publications below

Informations sur le produit

11508-1-AP cible PTP4A1/PRL1 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTP4A1/PRL1 Protéine recombinante Ag2067
• Nom complet: protein tyrosine phosphatase type IVA, member 1
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 18-20 kDa
• Numéro d’acquisition GenBank: BC023975
• Symbole du gène: PTP4A1
• Identification du gène (NCBI): 7803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTP4A1(protein tyrosine phosphatase type IVA, member 1), also named as PRL1, HH72, PRL-1, PTP(CAAX1), PTPCAAX1, DKFZp779M0721, belongs to the protein-tyrosine phosphatase family, which play an important role in maintaining appropriate tyrosine phosphorylation levels in the cell during development and tissue regeneration (PMID:19341304). PTP4A1 is a PTPase and that it is highly expressed in growing hepatic cells and some tumor cell lines and is located primarily in the cell nucleus, and its overexpression modulates the growth of cells (PMID:8196618). PTP4A1 can exist as dimer, trimer and higher oligomers in vivo (PMID: 15571731, 18224294).

Publications

Species	Application	Title
mouse	WB	Sci Signal; The adaptor SH2B1 and the phosphatase PTP4A1 regulate the phosphorylation of cytohesin-2 in myelinating Schwann cells in mice.; Authors - Yuki Miyamoto
human	WB	Oncotarget; Oncogenic ALK regulates EMT in non-small cell lung carcinoma through repression of the epithelial splicing regulatory protein 1.; Authors - Claudia Voena
human	WB,IHC	Int J Oncol; MicroRNA-1271 functions as a metastasis and epithelial-mesenchymal transition inhibitor in human HCC by targeting the PTP4A1/c-Src axis.; Authors - Chao Li ; KD Validated
human	WB	PLoS One; MiR-339-5p regulates the growth, colony formation and metastasis of colorectal cancer cells by targeting PRL-1.; Authors - Zhou Chang C
human	WB	Oncol Lett; miR-339-3p inhibits proliferation and metastasis of colorectal cancer.; Authors - Chang Zhou
human	WB,IHC	Int J Cancer; ERα inhibits mesenchymal and amoeboidal movement of liver cancer cell via Gα12; Authors - Jessica Yun ; KD Validated