Anticorps Polyclonal de lapin anti-PRL3

PRL3 Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15186-1-AP

Synonymes

PRL 3, PRL R, PRL3, PTP4A3

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Galerie de données de validation

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  WB analysis using 15186-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15186-1-AP (PRL3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 15186-1-AP (PRL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15186-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 15186-1-AP (PTP4A3 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu cardiaque humain, tissu de cancer de l'œsophage humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15186-1-AP cible PRL3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PRL3 Protéine recombinante Ag7249
• Nom complet: protein tyrosine phosphatase type IVA, member 3
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC003105
• Symbole du gène: PTP4A3
• Identification du gène (NCBI): 11156
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Phosphatase of regenerating liver-3 (PRL-3; also known as PTP4A3), encodes a 22-kDa protein that represents a novel class of a superfamily of protein tyrosine phosphatases (PTPs) that are ubiquitously expressed in various tumors. PRL3 was associated with primary tumor growth, tumor reoccurrence, and therapy resistance in many human cancers. This protein was also suggested as a marker to predict the relapse of gastric cancer and invasive breast cancer. (PMID: 22469786, PMID: 30157875)

Publications

Species	Application	Title
human	IHC	Reprod Sci; PRL-3 Is Involved in Estrogen- and IL-6-Induced Migration of Endometrial Stromal Cells From Ectopic Endometrium.; Authors - Shifan Ren
human	IF	Mol Cancer; Super-enhancer-driven TOX2 mediates oncogenesis in Natural Killer/T Cell Lymphoma; Authors - Jianbiao Zhou