Anticorps Polyclonal de lapin anti-PTPIP51

• Phare
• Validé par KD/KO

PTPIP51 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IHC, IF, IP, COIP, ELISA

Conjugaison

Non conjugué

Publications(22)

Avis (1)

N° de cat : 20641-1-AP

Synonymes

FAM82A2, Protein FAM82A2, hucep-10, hRMD-3, hRMD 3

---

Galerie de données de validation

• 

  WB analysis using 20641-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20641-1-AP (PTPIP51 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 20641-1-AP

  IP result of anti-PTPIP51 (IP:20641-1-AP, 4ug; Detection:20641-1-AP 1:1000) with HEK-293 cells lysate 1100ug.

• 

  IHC staining of human skin using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20641-1-AP (PTPIP51 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 20641-1-AP (PTPIP51 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human paracancerous of skin cancer using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human paracancerous of skin cancer slide using 20641-1-AP (PTPIP51 antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human cervical cancer using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20641-1-AP (PTPIP51 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human cervical cancer using 20641-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20641-1-AP (PTPIP51 antibody) at dilution of 1:100 (under 40x lens).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules Jurkat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: human paracancerous of skin cancer, tissu cutané humain, tissu de cancer du col de l'utérus humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 15 publications below
• IHC: See 2 publications below
• IF: See 9 publications below
• CoIP: See 2 publications below

Informations sur le produit

20641-1-AP cible PTPIP51 dans les applications de WB, IHC, IF, IP, COIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPIP51 Protéine recombinante Ag14708
• Nom complet: family with sequence similarity 82, member A2
• Masse moléculaire calculée: 470 aa, 52 kDa
• Poids moléculaire observé: 52-60 kDa
• Numéro d’acquisition GenBank: BC008970
• Symbole du gène: PTPIP51
• Identification du gène (NCBI): 55177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein tyrosine phosphatase-interacting protein 51 (PTPIP51),also known as RMDN3, is a mitochondrial membrane-anchored protein that interacts with ER membrane-bound VAPB. The VAPB-PTPIP51 interaction impacts on intracellular Ca2+ handling (PMID: 22131369, 28345618). PTPIP51 participates in multiple cellular processes, and dysfunction of PTPIP51 is implicated in diseases such as cancer and neurodegenerative disorders (PMID: 28345618). The known initiation sites in the Ptpip51 gene would lead to molecular protein masses of 52, 38 and 30 kDa (PMID: 18854601,19844996).

Publications

Species	Application	Title
human	WB	Cell Metab; A High-Density Human Mitochondrial Proximity Interaction Network.; Authors - Hana Antonicka
monkey	WB,IF	Nat Cell Biol; MIROs and DRP1 drive mitochondrial-derived vesicle biogenesis and promote quality control.; Authors - Tim König ; KD Validated
human	COIP	Mol Cell; The ER-Localized Transmembrane Protein EPG-3/VMP1 Regulates SERCA Activity to Control ER-Isolation Membrane Contacts for Autophagosome Formation.; Authors - Yan G Zhao
human	WB	J Cell Biol; Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.; Authors - Nan Liu
human	WB,CoIP	EMBO Rep; Nup358 restricts ER-mitochondria connectivity by modulating mTORC2/Akt/GSK3β signalling; Authors - Misha Kalarikkal ; KD Validated
human	WB	J Cell Sci; The role of Mfn2 in the structure and function of endoplasmic reticulum-mitochondrial tethering in vivo.; Authors - Song Han

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Tom (Verified Customer) (03-23-2021)

Cos7 cells fixed in 4% PFA, perm in 0.1% Triton then blocked in 1% BSA. Mitochondria also labelled with TOMM20. Cells incubated with primary antibody (1/100) for 1 hour.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1/100
• Cell Tissue Type: Cos7

PTPIP51 Antibody Immunofluorescence validation (1/100 dilution) in Cos7 (Cat no:20641-1-AP)

{{ptg:RelatedPrimaryAntibodies}}