Anticorps Polyclonal de lapin anti-PTP1B

• Phare
• Validé par KD/KO

PTP1B Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

29

N° de cat : 11334-1-AP

Synonymes

PTP 1B, PTP1B, PTPN1

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Galerie de données de validation

• 

  WB analysis of Jurkat using 11334-1-AP

  WB result of PTP1B antibody (11334-1-AP; 1:30000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTP1B transfected Jurkat cells.

• 

  WB analysis of Sp2/0 using 11334-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 11334-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 11334-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 11334-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11334-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of BxPC-3 using 11334-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11334-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 11334-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 11334-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11334-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11334-1-AP (PTP1B antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A431 using 11334-1-AP

  IP Result of anti-PTP1B (IP:11334-1-AP, 4ug; Detection:11334-1-AP 1:500) with A431 cells lysate 2400ug.

• 

  IHC staining of human prostate cancer using 11334-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 11334-1-AP (PTP1B antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of BxPC-3 using 11334-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 11334-1-AP (PTP1B antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Sp2/0, cellules A431, cellules BxPC-3, cellules C6, cellules HeLa, cellules HL-60, cellules Jurkat, cellules NIH/3T3, cellules THP-1, tissu hépatique de rat, tissu hépatique de souris, tissu placentaire de souris, tissu placentaire humain, tissu testiculaire humain
• Résultats positifs en IP: cellules A431
• Résultats positifs en IHC: tissu de cancer de la prostate humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 28 publications below
• IHC: See 7 publications below
• IF: See 6 publications below
• IP: See 2 publications below
• CoIP: See 2 publications below

Informations sur le produit

11334-1-AP cible PTP1B dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTP1B Protéine recombinante Ag1878
• Nom complet: protein tyrosine phosphatase, non-receptor type 1
• Masse moléculaire calculée: 435 aa, 50 kDa
• Poids moléculaire observé: 45-55 kDa
• Numéro d’acquisition GenBank: BC015660
• Symbole du gène: PTPN1
• Identification du gène (NCBI): 5770
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN1 (protein tyrosine phosphatase, non-receptor type 1) is also named as PTP1B or PTP-1B, and belongs to the protein-tyrosine phosphatase family and non-receptor class 1 subfamily. PTPN1 is involved in the regulation of several important physiological pathways, which regulates both INS and leptin signaling, and interacts with the epidermal-and platelet-derived growth factor receptors (PMID: 20101100). The gene might harbor variants influencing susceptibility to INS resistance and type 2 diabetes (PMID:15919813). The molecular mass of PTPN1 is 50 kDa, but sometimes some truncated forms can be detected as 48, 46, 39, 37 kDa due to non-specific proteolytic cleavage of PTP1B in different cells (PMID: 18253097).

Publications

Species	Application	Title
human	WB	Aging (Albany NY); Glycochenodeoxycholic acid induces stemness and chemoresistance via the STAT3 signaling pathway in hepatocellular carcinoma cells.; Authors - Changying Shi
rat	WB	J Cell Physiol; Circular RNA circTET3 mediates migration of rat vascular smooth muscle cells by targeting miR-351-5p.; Authors - Qing-Ping Yao
mouse	WB,IHC	FASEB J; PMID: 32100381; Authors - Ping Yang
human	WB	Front Cell Dev Biol; Tubeimoside I Inhibits Cell Proliferation and Induces a Partly Disrupted and Cytoprotective Autophagy Through Rapidly Hyperactivation of MEK1/2-ERK1/2 Cascade via Promoting PTP1B in Melanoma.; Authors - Juan Du ; KD Validated
mouse	WB	Eur J Pharmacol; Oleanolic acid derivative alleviates cardiac fibrosis through inhibiting PTP1B activity and regulating AMPK/TGF-β/Smads pathway; Authors - An-Hui Wang
mouse	WB	iScience; Neuronal XRN1 is required for maintenance of whole-body metabolic homeostasis; Authors - Shohei Takaoka