Anticorps Polyclonal de lapin anti-PTPN22

PTPN22 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (2)

Applications

WB, IHC, IF, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 11783-1-AP

Synonymes

EC:3.1.3.48, Hematopoietic cell protein-tyrosine phosphatase 70Z-PEP, LyP, LYP1, LYP2

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Galerie de données de validation

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  WB analysis using 11783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 11783-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 11783-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11783-1-AP (PTPN22 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of Jurkat using 11783-1-AP

  IP result of anti-PTPN22 (IP:11783-1-AP, 4ug; Detection:11783-1-AP 1:300) with Jurkat cells lysate 3080ug.

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  IHC staining of human lymphoma using 11783-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11783-1-AP (PTPN22 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules Jurkat, cellules Raji
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de lymphome humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

11783-1-AP cible PTPN22 dans les applications de WB, IHC, IF, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPN22 Protéine recombinante Ag2397
• Nom complet: protein tyrosine phosphatase, non-receptor type 22 (lymphoid)
• Masse moléculaire calculée: 82 kDa, 92 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC017785
• Symbole du gène: PTPN22
• Identification du gène (NCBI): 26191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPN22(Tyrosine-protein phosphatase non-receptor type 22) is also named as LyP,PEP,PTPN8.It belongs to the protein-tyrosine phosphatase family and non-receptor class 4 subfamily.It acts as negative regulator of T-cell receptor (TCR) signaling.Defects in PTPN22 are a cause of susceptibility to systemic lupus erythematosus (SLE). It has some isoforms produced by alternative splicing with calculated molecular mass of 79-92 kDa, and apparent molecular mass of 100-110 kDa.

Publications

Species	Application	Title
mouse	WB	J Exp Med; NLR Nod1 signaling promotes survival of BCR-engaged mature B cells through up-regulated Nod1 as a positive outcome.; Authors - Kyoko Hayakawa
mouse	WB，IP	Sci Signal; The adaptor protein TRAF3 inhibits interleukin-6 receptor signaling in B cells to limit plasma cell development.; Authors - Wai W Lin
human	WB	Sci Rep; TRAF3 enhances TCR signaling by regulating the inhibitors Csk and PTPN22.; Authors - Alicia M Wallis
rat	IF	Pflugers Arch; Anandamide produced by Ca(2+)-insensitive enzymes induces excitation in primary sensory neurons.; Authors - Varga Angelika A
rat	WB,IHC,CoIP	J Adv Res; PTPN22 as a therapeutic target in intervertebral disc degeneration: Modulating mitophagy and pyroptosis through the PI3K/AKT/mTOR axis; Authors - Haibo Liang

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