Anticorps Polyclonal de lapin anti-IA-2/PTPRN

IA-2/PTPRN Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10584-1-AP

Synonymes

IA 2, IA 2/PTP, IA2, ICA 512, ICA3, ICA512, Islet cell antigen 512, Islet cell autoantigen 3, PTP IA 2, PTPRN, R PTP N

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Galerie de données de validation

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  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10584-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 10584-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10584-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10584-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10584-1-AP

  IP Result of anti-IA-2/PTPRN (IP:10584-1-AP, 3ug; Detection:10584-1-AP 1:600) with mouse brain tissue lysate 7000ug.

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  IHC staining of human pancreas cancer using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 10584-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 10584-1-AP (IA-2/PTPRN antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of mouse pancreas using 10584-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse pancreas tissue using IA-2/PTPRN antibody (10584-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of HEK-293 using 10584-1-AP

  1X10^6 HEK-293 cells were intracellularly stained with 0.4 ug Anti-Human IA-2/PTPRN (10584-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of Y79 using 10584-1-AP

  1X10^6 Y79 cells were intracellularly stained with 0.4 ug Anti-Human IA-2/PTPRN (10584-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat, tissu rénal de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du pancréas humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu pancréatique de souris,
• Résultats positifs en cytométrie: cellules HEK-293, cellules Y79

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below

Informations sur le produit

10584-1-AP cible IA-2/PTPRN dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: IA-2/PTPRN Protéine recombinante Ag0923
• Nom complet: protein tyrosine phosphatase, receptor type, N
• Masse moléculaire calculée: 105 kDa
• Poids moléculaire observé: 60-65 kDa, 106-115 kDa
• Numéro d’acquisition GenBank: BC007713
• Symbole du gène: PTPRN
• Identification du gène (NCBI): 5798
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

IA-2/PTPRN is a member of the protein tyrosine phosphatase (PTP) family, which contains a transmembrane region, an intracellular PTP-like domain and an extracellular N-terminus. Experiments found that IA-2/PTPRN localizes to secretory granules and is exclusively expressed in neuroendocrine cells (including pancreatic islets cell).(PMID: 10027571). IA-2/PTPRN was found to be a major autoantigen in insulin-dependent diabetes mellitus and the detection of autoantibodies against IA-2/PTPRN is commonly used as a diabetes diagnosis marker.

Publications

Species	Application	Title
human	WB,IHC	Front Cell Dev Biol; Overexpression of PTPRN Promotes Metastasis of Lung Adenocarcinoma and Suppresses NK Cell Cytotoxicity.; Authors - Xinyue Song
human	WB	J Biol Chem; Down-regulation of the islet-specific zinc transporter-8 (ZnT8) protects human insulinoma cells against inflammatory stress.; Authors - Chengfeng Merriman
		PLoS One; Sensitive detection of multiple islet autoantibodies in type 1 diabetes using small sample volumes by agglutination-PCR.; Authors - Felipe de Jesus Cortez
human	IHC	Cell Physiol Biochem; Prognostic Value of Phosphotyrosine Phosphatases in Hepatocellular Carcinoma.; Authors - Guangyan Zhangyuan