Anticorps Polyclonal de lapin anti-PTPRR

PTPRR Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 17937-1-AP

Synonymes

Ch 1PTPase, EC PTP, ECPTP, NC PTPCOM1, PCPTP1, PTP SL, PTPBR7, PTPRQ, PTPRR, R PTP R

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Galerie de données de validation

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  WB analysis using 17937-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 17937-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 17937-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 17937-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 17937-1-AP (PTPRR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COLO 320, cellules SH-SY5Y, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

17937-1-AP cible PTPRR dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPRR Protéine recombinante Ag12145
• Nom complet: protein tyrosine phosphatase, receptor type, R
• Masse moléculaire calculée: 657 aa, 74 kDa
• Poids moléculaire observé: 32 kDa, 47 kDa, 65 kDa
• Numéro d’acquisition GenBank: BC110900
• Symbole du gène: PTPRR
• Identification du gène (NCBI): 5801
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PTPRR(protein tyrosine phosphatase, receptor type, R) is also named as PCPTP1, PTP-SL, PTPBR7 and belongs to the protein-tyrosine phosphatase family. It sequesters mitogen-activated protein kinases (MAPKs) such as MAPK1, MAPK3 and MAPK14 in the cytoplasm in an inactive form. It has four isoforms that they have molecular weights of 72, 60, 42 and 37 kDa, respectively, and differ in the length of their N-terminal part which result in distinct subcellular localizations. All PTPRR isoforms are found to be short-lived proteins, with half-lives of around 3-5 hours, and are constitutively phosphorylated on one or two of the previously identified protein kinase A and MAP kinase target sites.

Publications

Species	Application	Title
mouse	WB	J Affect Disord; PTPRR regulates ERK dephosphorylation in depression mice model.; Authors - Xinrong Li
human	WB	BMC Cancer; Androgen-regulation of the protein tyrosine phosphatase PTPRR activates ERK1/2 signalling in prostate cancer cells.; Authors - Jennifer Munkley
mouse	WB,IF	Front Mol Neurosci; Downregulation of Protein Tyrosine Phosphatase Receptor Type R Accounts for the Progression of Hirschsprung Disease.; Authors - Jiao Tian