Anticorps Polyclonal de lapin anti-PTPRS

• Phare
• Validé par KD/KO

PTPRS Polyclonal Antibody for FC, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 13008-1-AP

Synonymes

PTPRS, PTPSIGMA, R PTP S, R PTP sigma, rPTPσ

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Galerie de données de validation

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  WB analysis of HeLa using 13008-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13008-1-AP (PTPRS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13008-1-AP

  WB result of PTPRS antibody (13008-1-AP; 1:500; incubated at room temperature for 1.5 hours) with sh-Control and sh-PTPRS transfected HeLa cells.

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  WB analysis of mouse brain using 13008-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13008-1-AP (PTPRS antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13008-1-AP

  IP Result of anti-PTPRS (IP:13008-1-AP, 5ug; Detection:13008-1-AP 1:400) with mouse brain tissue lysate 6500ug.

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  IHC staining of human kidney using 13008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13008-1-AP (PTPRS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 13008-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 13008-1-AP (PTPRS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of HeLa using 13008-1-AP

  1X10^6 HeLa cells were stained with 0.2ug PTPRS antibody (13008-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu rénal humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en cytométrie: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

13008-1-AP cible PTPRS dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PTPRS Protéine recombinante Ag3691
• Nom complet: protein tyrosine phosphatase, receptor type, S
• Masse moléculaire calculée: 128aa,14 kDa; 1948aa,217 kDa
• Poids moléculaire observé: 75-100 kDa
• Numéro d’acquisition GenBank: BC029496
• Symbole du gène: PTPRS
• Identification du gène (NCBI): 5802
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type II a receptor protein tyrosine phosphatases (rPTPσ) are cell surface receptors important for nervous system development, function, and repair.The expression of rPTPσ has previously been reported in b-cells and other target organs for INS although the probes chosen did not permit to distinguish between the splice variants.Proteolytic processing near the transmembrane domain generates an extracellular N-terminal E-domain of 130 kDa and a C-terminal P-domain of approximately 85 kDa of rPTPσ,and the short splice variants rPTPσ 3 and 4 contain an E-domain of 95 kDa (PMID: 16552719). rPTPσ expression was observed in tissue lysates of the adult mouse sensory-motor cortex and thoracic spinal cord (T8-T10) as a 75-80kDa immunoreactive band (PMID: 19780196).

Publications

Species	Application	Title
mouse	WB	Elife; Synaptic plasticity and cognitive function are disrupted in the absence of Lrp4.; Authors - Andrea M Gomez
human	WB	ACS Chem Neurosci; Small Molecules Targeting PTPσ-Trk Interactions Promote Sympathetic Nerve Regeneration.; Authors - Matthew R Blake
human	WB	J Gene Med; Downregulation of lncRNA HCG11 promotes cell proliferation of oral squamous cell carcinoma through sponging miR-455-5p.; Authors - Jingjing Wu
rat	WB,IHC	Curr Eye Res; Expression of Perineuronal Nets, Parvalbumin and Protein Tyrosine Phosphatase σ in the Rat Visual Cortex During Development and After BFD.; Authors - Liu Hui H
mouse	IF,WB	J Cell Biochem; Chondroitin sulfate proteoglycan represses neural stem/progenitor cells migration via PTPσ/α-actinin4 signaling pathway.; Authors - Jun Zhong