Anticorps Polyclonal de lapin anti-PURA

• Phare
• Validé par KD/KO

PURA Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 17733-1-AP

Synonymes

PUR ALPHA, PUR1, PURA, PURALPHA

---

Galerie de données de validation

• 

  WB analysis using 17733-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17733-1-AP (PURA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 17733-1-AP

  WB result of PURA antibody (17733-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PURA transfected HepG2 cells.

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules HepG2, cellules MG U-87

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

17733-1-AP cible PURA dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PURA Protéine recombinante Ag12173
• Nom complet: purine-rich element binding protein A
• Masse moléculaire calculée: 322 aa, 35 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC036087
• Symbole du gène: PURA
• Identification du gène (NCBI): 5813
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PURA, also named as Transcriptional activator protein Pur-alpha, is a 322 amino acid protein, which belongs to the PUR DNA-binding protein family and can form homodimer or heterodimer with PURB and heterotrimer with PURB and YBX1/Y-box protein 1. PURA is a probable transcription activator that specifically binds the purine-rich single strand of the PUR element located upstream of the MYC gene and may play a role in the initiation of DNA replication and in recombination.

Publications

Species	Application	Title
human	RIP	Nucleic Acids Res; Corrigendum to article "Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation''.; Authors - Ruibing Chen
human	WB,IHC,IF,IP	Cancer Res; LncRNA AGPG confers endocrine resistance in breast cancer by promoting E2F1 activity; Authors - Shiyi Yu
human	WB,IP	EBioMedicine; Genome-wide CRISPR/Cas9 screening identifies a targetable MEST-PURA interaction in cancer metastasis; Authors - Wen Wen Xu ; KD Validated
human	WB	Nucleic Acids Res; Quantitative proteomics reveals that long non-coding RNA MALAT1 interacts with DBC1 to regulate p53 acetylation.; Authors - Ruibing Chen
rat	WB	Front Mol Neurosci; YAP and TAZ Regulate Cc2d1b and Purβ in Schwann Cells.; Authors - Belin Sophie
rat	WB	Acta Histochem; Protective effects of astaxanthin on subarachnoid hemorrhage-induced early brain injury: Reduction of cerebral vasospasm and improvement of neuron survival and mitochondrial function.; Authors - Yanbin Wang